Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed (ab6267)
Key features and details
- Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ELISA, Immunomicroscopy, Flow Cyt
Overview
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Product name
Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins. -
Tested applications
Suitable for: ICC/IF, ELISA, Immunomicroscopy, Flow Cytmore details -
Minimal
cross-reactivity
Cow, Horse, Human more details -
Immunogen
Rat IgG F(ab’)2 fragment
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Conjugation
Texas Red ®. Ex: 596nm, Em: 620nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 1.46% Sodium chloride, 1% BSA, 0.164% Sodium phosphate
BSA is IgG and protease free -
Concentration information loading... -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.5 moles Texas Red per mole of Goat IgG F(ab’)2 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| ELISA | ||
| Immunomicroscopy | ||
| Flow Cyt |
| Notes |
|---|
Application notes
Flow Cyt: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab6267 has been referenced in 9 publications.
- Xiao H et al. Metformin ameliorates bleomycin-induced pulmonary fibrosis in mice by suppressing IGF-1. Am J Transl Res 12:940-949 (2020). PubMed: 32269725
- Zheng H et al. MicroRNA-138 Inhibits Osteogenic Differentiation and Mineralization of Human Dedifferentiated Chondrocytes by Regulating RhoC and the Actin Cytoskeleton. JBMR Plus 3:e10071 (2019). PubMed: 30828688
- Zheng H et al. MicroRNA-181a/b-1 over-expression enhances osteogenesis by modulating PTEN/PI3K/AKT signaling and mitochondrial metabolism. Bone 123:92-102 (2019). PubMed: 30898695
- Li B et al. Ouabain ameliorates bleomycin induced pulmonary fibrosis by inhibiting proliferation and promoting apoptosis of lung fibroblasts. Am J Transl Res 10:2967-2974 (2018). PubMed: 30323883
- Xing W et al. Amantadine attenuates sepsis-induced cognitive dysfunction possibly not through inhibiting toll-like receptor 2. J Mol Med (Berl) 96:391-402 (2018). PubMed: 29502203
- Zhu C et al. Phytophthora capsici homologue of the cell cycle regulator SDA1 is required for sporangial morphology, mycelial growth and plant infection. Mol Plant Pathol 17:369-87 (2016). PubMed: 26095317
- Zhou X et al. Antiaging Gene Klotho Regulates Adrenal CYP11B2 Expression and Aldosterone Synthesis. J Am Soc Nephrol 27:1765-76 (2016). PubMed: 26471128
- Louis ED et al. Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex. Neurosci Lett 518:49-54 (2012). PubMed: 22561033
- Ørom UA et al. MicroRNA-10a binds the 5'UTR of ribosomal protein mRNAs and enhances their translation. Mol Cell 30:460-71 (2008). PubMed: 18498749