Product nameGoat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed
See all IgG secondary antibodies
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
Tested applicationsSuitable for: ICC/IF, ELISA, Immunomicroscopy, Flow Cytmore details
Cow, Horse, HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Rat IgG F(ab’)2 fragment
ConjugationTexas Red ®. Ex: 596nm, Em: 620nm
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 1.46% Sodium chloride, 1% BSA, 0.164% Sodium phosphate
BSA is IgG and protease free
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesTexas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.5 moles Texas Red per mole of Goat IgG F(ab’)2
Our Abpromise guarantee covers the use of ab6267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This product has been referenced in:
- Zheng H et al. MicroRNA-181a/b-1 over-expression enhances osteogenesis by modulating PTEN/PI3K/AKT signaling and mitochondrial metabolism. Bone 123:92-102 (2019). Read more (PubMed: 30898695) »
- Zheng H et al. MicroRNA-138 Inhibits Osteogenic Differentiation and Mineralization of Human Dedifferentiated Chondrocytes by Regulating RhoC and the Actin Cytoskeleton. JBMR Plus 3:e10071 (2019). Read more (PubMed: 30828688) »