Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed (ab6267)

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Overview

  • Product name

    Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rat

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.

  • Tested applications

    Suitable for: ICC/IF, ELISA, Immunomicroscopy, Flow Cytmore details

  • Minimal
    cross-reactivity


    Cow, Horse, Human more details

  • Immunogen

    Rat IgG F(ab’)2 fragment

  • Conjugation

    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 1.46% Sodium chloride, 1% BSA, 0.164% Sodium phosphate

    BSA is IgG and protease free
  • Concentration information loading...

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • Conjugation notes

    Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.5 moles Texas Red per mole of Goat IgG F(ab’)2

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
Immunomicroscopy
Flow Cyt
  • Application notes
    Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References

    This product has been referenced in:

    • Zheng H  et al. MicroRNA-181a/b-1 over-expression enhances osteogenesis by modulating PTEN/PI3K/AKT signaling and mitochondrial metabolism. Bone 123:92-102 (2019). Read more (PubMed: 30898695) »
    • Zheng H  et al. MicroRNA-138 Inhibits Osteogenic Differentiation and Mineralization of Human Dedifferentiated Chondrocytes by Regulating RhoC and the Actin Cytoskeleton. JBMR Plus 3:e10071 (2019). Read more (PubMed: 30828688) »
    See all 7 Publications for this product

    Publishing research using ab6267? Please let us know so that we can cite the reference in this datasheet.

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