Key features and details
- Goat F(ab')2 Anti-Rat IgG - Fc (AP), pre-adsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC
Product nameGoat F(ab')2 Anti-Rat IgG - Fc (AP), pre-adsorbed
See all IgG secondary antibodies
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG, Rat IgG Fc and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat F(ab’)2 or Bovine, Horse, and Human Serum Proteins.
Tested applicationsSuitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details
Cow, Horse, HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Rat IgG F(c) fragment
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.00
Preservative: 0.01% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Our Abpromise guarantee covers the use of ab6258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/1000 - 1/5000.
IHC: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1000 to 1/5000 of the reconstitution concentration is suggested for this product.
Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6258 has not yet been referenced specifically in any publications.