Goat F(ab')2 Anti-Rat IgG - Fc, pre-adsorbed (ab6252)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Goat F(ab')2 Anti-Rat IgG - Fc, pre-adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins. -
Tested applications
Suitable for: WB, ICC/IF, ELISAmore details -
Minimal
cross-reactivity
Cow, Horse, Human more details -
Immunogen
Rat IgG F(c) fragment
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
Our Abpromise guarantee covers the use of ab6252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/5000. | |
| ICC/IF | 1/1000 - 1/3000. | |
| ELISA | 1/10000 - 1/50000. |
Datasheets and documents
References
This product has been referenced in:
- Gomez AM et al. Proteasome inhibition with bortezomib depletes plasma cells and autoantibodies in experimental autoimmune myasthenia gravis. J Immunol 186:2503-13 (2011). Sandwich ELISA . Read more (PubMed: 21239719) »
