Goat F(ab')2 Anti-Rat IgG - Fc, pre-adsorbed (ab6252)

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Key features and details

  • Goat F(ab')2 Anti-Rat IgG - Fc, pre-adsorbed
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, ICC/IF, ELISA

Overview

  • Product name

    Goat F(ab')2 Anti-Rat IgG - Fc, pre-adsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rat

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins.

  • Tested applications

    Suitable for: WB, ICC/IF, ELISAmore details

  • Minimal
    cross-reactivity


    Cow, Horse, Human more details

  • Immunogen

    Rat IgG F(c) fragment

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6252 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/2000 - 1/5000.
    ICC/IF
    1/1000 - 1/3000.
    ELISA
    1/10000 - 1/50000.
    Notes
    WB
    1/2000 - 1/5000.
    ICC/IF
    1/1000 - 1/3000.
    ELISA
    1/10000 - 1/50000.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab6252? Please let us know so that we can cite the reference in this datasheet.

    ab6252 has been referenced in 1 publication.

    • Gomez AM  et al. Proteasome inhibition with bortezomib depletes plasma cells and autoantibodies in experimental autoimmune myasthenia gravis. J Immunol 186:2503-13 (2011). Sandwich ELISA . PubMed: 21239719

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