Overview

  • Product name
    Goat F(ab')2 Anti-Rat IgG - Fc, pre-adsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rat
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins.
  • Tested applications
    Suitable for: WB, ICC/IF, ELISAmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Rat IgG F(c) fragment

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000.
ICC/IF 1/1000 - 1/3000.
ELISA 1/10000 - 1/50000.

References

This product has been referenced in:
  • Gomez AM  et al. Proteasome inhibition with bortezomib depletes plasma cells and autoantibodies in experimental autoimmune myasthenia gravis. J Immunol 186:2503-13 (2011). Sandwich ELISA . Read more (PubMed: 21239719) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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