Overview

  • Product name
    Goat F(ab')2 Anti-Rat IgG - H&L (FITC), pre-adsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rat
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Rat IgG whole molecule

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.2 moles FITC per mole of Goat IgG F(ab’)2
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000. PubMed:17173070
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

References

This product has been referenced in:
  • Jiang H  et al. Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer Vaccination. Cancer Res 77:3894-3907 (2017). Read more (PubMed: 28566332) »
  • Giannakara A  et al. Sperm competition-induced plasticity in the speed of spermatogenesis. BMC Evol Biol 16:60 (2016). IHC - Wholemount . Read more (PubMed: 26956948) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

I'm sorry to hear the customer is experiencing problems in FACS with some of our antibodies, it is likely that the problem is protocol related. I have received this morning a detailed FACS protocol with details of the buffers to use and fixative to use for staining with ab17323 and am still waiting for this information from the source of the anti TAC1 antibody, my apologies. The positive control recommended for endogenous detection by FACS is U266 cells. Recommended FACS protocol with ab17323 Buffers: PBS 1% Formaldehyde (FA) keep at 4°C PBS 0.5% saponin keep at 4°C PBS 50 mM NH4Cl 1.33g NH4Cl for 500ml Complete medium 0.5% saponin 3 x 105 cells/sample Protocol 1-wash cells 1X in PBS 2-incubate in 0.5 ml PBS 1%FA, 15 min at RT under agitation 3-wash 1X with PBS 4-wash 1X with PBS-NH4Cl 5-wash 1X with PBS 6-incubate with the first antibody in complete medium + 0.5% saponin for 30 min at RT. 7-wash once with 1X PBS 0.5% saponin 8-incubate with the secondary antibody in complete medium + 0.5% saponin for 30 min (or more depending on secondary specification) at RT in the dark. 9-wash once with PBS 0.5% saponin 10-wash once with PBS 11-resuspend in 0.2 ml PBS 12- analyse with FACS I hope this information helps,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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