Goat Anti-Ferret IgG H&L (HRP) (ab112770)
Key features and details
- Goat Anti-Ferret IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ELISA, IHC-P, ICC/IF
Related conjugates and formulations
Overview
-
Product name
Goat Anti-Ferret IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Ferret -
Specificity
By immunoelectrophoresis and ELISA ab112770 reacts specifically with Ferret IgG and with light chains common to other Ferret immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. May cross react with IgG from other species. -
Tested applications
Suitable for: WB, ELISA, IHC-P, ICC/IFmore details -
Immunogen
Other Immunogen Type corresponding to IgG.
-
Conjugation
HRP
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab112770 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). -
Conjugation notes
Molar enzyme/antibody protein ratio is 4:1. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. colorimetric detection; 1/1000 - 1/30000 chemiluminescent detection.
|
|
ELISA |
1/10000 - 1/100000. as a primary.
|
|
IHC-P |
1/200 - 1/500.
|
|
ICC/IF |
1/200 - 1/500.
|
Notes |
---|
WB
1/1000 - 1/10000. colorimetric detection; 1/1000 - 1/30000 chemiluminescent detection. |
ELISA
1/10000 - 1/100000. as a primary. |
IHC-P
1/200 - 1/500. |
ICC/IF
1/200 - 1/500. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (10)
ab112770 has been referenced in 10 publications.
- Kassardjian A et al. Modular adjuvant-free pan-HLA-DR-immunotargeting subunit vaccine against SARS-CoV-2 elicits broad sarbecovirus-neutralizing antibody responses. Cell Rep 42:112391 (2023). PubMed: 37053069
- Park J et al. An inactivated multivalent influenza A virus vaccine is broadly protective in mice and ferrets. Sci Transl Med 14:eabo2167 (2022). PubMed: 35857640
- Pulit-Penaloza JA et al. Comparative Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Variants in the Ferret Model. mBio 13:e0242122 (2022). PubMed: 36135377
- Vilas Boas de Melo C et al. Influenza Infection in Ferrets with SARS-CoV-2 Infection History. Microbiol Spectr 10:e0138622 (2022). PubMed: 36301107
- Le Sage V et al. Pre-existing heterosubtypic immunity provides a barrier to airborne transmission of influenza viruses. PLoS Pathog 17:e1009273 (2021). PubMed: 33600489
- Kutter JS et al. SARS-CoV and SARS-CoV-2 are transmitted through the air between ferrets over more than one meter distance. Nat Commun 12:1653 (2021). PubMed: 33712573
- Boyoglu-Barnum S et al. Quadrivalent influenza nanoparticle vaccines induce broad protection. Nature 592:623-628 (2021). PubMed: 33762730
- Pavel STI et al. Development of an Inactivated Vaccine against SARS CoV-2. Vaccines (Basel) 9:N/A (2021). PubMed: 34835197
- Richard M et al. SARS-CoV-2 is transmitted via contact and via the air between ferrets. Nat Commun 11:3496 (2020). PubMed: 32641684
- Meyerson NR et al. Nuclear TRIM25 Specifically Targets Influenza Virus Ribonucleoproteins to Block the Onset of RNA Chain Elongation. Cell Host Microbe 22:627-638.e7 (2017). PubMed: 29107643