Product nameGoat Anti-Guinea pig IgG H&L (Alexa Fluor® 405)
See all IgG secondary antibodies
Target speciesGuinea pig
SpecificityThis antibody is specific to Guinea pig IgG
Tested applicationsSuitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
The details of the immunogen for this antibody are not available.
ConjugationAlexa Fluor® 405. Ex: 402nm, Em: 421nm
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. Store In the Dark.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 23% Glycerol, PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Use goat anti-guinea pig IgG (H&L) Alexa Fluor® 405 to confidently detect primary antibodies raised in guinea pig in your immunocytochemistry, flow cytometry or immunohistochemistry. Get dilutions to get the best results in your cell staining, flow cytometry or ELISA with goat anti-guinea pig IgG (H&L) Alexa Fluor® 405 below.
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Our Abpromise guarantee covers the use of ab175678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||1/200 - 1/1000.
We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below)
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The cells were then incubated with ab7075, rabbit anti-mouse HRP conjugated antibody, for 1h at a 1/250 dilution. The cells were then incubated with ab34580, guinea pig anti-HRP antibody, for 1h at a 1/250 dilution. Lastly, the secondary antibody (blue) was ab175678, Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405), used at 1µg/ml for 1h, as a quaternary antibody. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a dilution of 1/4000.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab175678 has been referenced in 5 publications.
- Rademacher N et al. Intramolecular domain dynamics regulate synaptic MAGUK protein interactions. Elife 8:N/A (2019). PubMed: 30864948
- Corbett BF et al. Sphingosine-1-phosphate receptor 3 in the medial prefrontal cortex promotes stress resilience by reducing inflammatory processes. Nat Commun 10:3146 (2019). PubMed: 31316053
- Lieb A et al. Biochemical autoregulatory gene therapy for focal epilepsy. Nat Med 24:1324-1329 (2018). PubMed: 29988123
- Kunde SA et al. Protein kinase C regulates AMPA receptor auxiliary protein Shisa9/CKAMP44 through interactions with neuronal scaffold PICK1. FEBS Open Bio 7:1234-1245 (2017). PubMed: 28904854
- Rademacher N et al. MPP2 is a postsynaptic MAGUK scaffold protein that links SynCAM1 cell adhesion molecules to core components of the postsynaptic density. Sci Rep 6:35283 (2016). ICC/IF . PubMed: 27756895