Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (ab150187)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647)
See all IgG secondary antibodies -
Description
Goat polyclonal Secondary Antibody to Guinea pig IgG - H&L (Alexa Fluor® 647) -
Host species
Goat -
Target species
Guinea pig -
Specificity
This antibody is specific to Guinea pig IgG -
Tested applications
Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol, PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Applications
Our Abpromise guarantee covers the use of ab150187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | 1/200 - 1/1000. | |
| Flow Cyt | 1/2000. | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. |
Images
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (ab150187)ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The cells were then incubated with ab7075, rabbit anti-mouse HRP conjugated antibody, for 1h at a 1/250 dilution. The cells were then incubated with ab34580, guinea pig anti-HRP antibody, for 1h at a 1/250 dilution. Lastly, the secondary antibody (red) was ab150187 Alexa Fluor® 647 goat anti-guinea pig IgG (H+L) used at 1µg/ml for 1h, as a quaternary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (ab150187)HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (red) was ab150187 Alexa Fluor® 647 goat anti-guinea pig IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (ab150187)
References
This product has been referenced in:
- Guida C et al. Sitagliptin and Roux-en-Y gastric bypass modulate insulin secretion via regulation of intra-islet PYY. Diabetes Obes Metab 20:571-581 (2018). Read more (PubMed: 28892258) »
- Sepsi A et al. ImmunoFISH: Simultaneous Visualisation of Proteins and DNA Sequences Gives Insight Into Meiotic Processes in Nuclei of Grasses. Front Plant Sci 9:1193 (2018). Read more (PubMed: 30154816) »
