Goat Anti-Guinea pig IgG H&L (Cy3.5 ®) preadsorbed (ab6966)

Overview

  • Product name
    Goat Anti-Guinea pig IgG H&L (Cy3.5 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Guinea pig IgG - H&L (Cy3.5 ®), pre-adsorbed
  • Host species
    Goat
  • Target species
    Guinea pig
  • Tested applications
    Suitable for: ICC/IF, ICC, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native Guinea pig IgG (purified).

  • Conjugation
    Cy3.5 ®. Ex: 581nm, Em: 596nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
  • Conjugation notes
    Cy3.5 (Cyanine 3.5-OSu) (Molecular Weight 1286 daltons) Absorption Wavelength: 581 nm Emission Wavelength: 596 nm Fluorochrome/Protein Ratio: 3.4 moles Cy3.5 per mole of Goat IgG
  • Clonality
    Polyclonal
  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
ICC Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

References

ab6966 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply! Indeed, firstly I wanted to suggest also to use Texas Red with TRITC. However when I looked closer at the emission spectra, it seemed to me that there is quite a "bleeding" of the TRITC into the Texas Red. However this depends also on the staining intensities and the filters available on the microscope. http://www.mcb.arizona.edu/ipc/fret/index.html The DyLight 594 however is still excited (to about 40%) with the laser at 542nm. I thought therefore this might be the safer option. https://www.abcam.com/ps/CMS/Images/DL_594_orange[1]378px302px.jpg It is however up to you to decide. Either option you will chose, we will continue to support you. We will also reward you with points for discounts/amazon vouchers if you can provide your feedback with an image. I hope this information is helpful. Please do not hesitate to contact us again should you have further questions. Merry Christmas and happy new Year to you as well!

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Answer

Thank you very much for your inquiry. Indeed we do have a number of antibodies which can be suitable for your application. Please find here below a selection. I have only proposed pre-absorbed antibodies, as they will help to reduce background in immunohistochemistry, especially when doing double stainings. We do also have other formulations of many of these antibodies. For example, we do have antibodies also produced in donkey, against the Fc region or as an F(ab')2. Please do let me know if you have any preference in these regards and I'll be happy to include them in the selection. I have excluded in this list fluorochromes who could interfere with the GFP staining. If this would not matter however, please let me know. Indeed, the request formulated is rather specific. The best option I can propose is, to use the Cy3.5 conjugated anti guinea pig ab6966, with the DyLight 594 conjugated anti rabbit antibody ab96921. I would expect this to provide a good double staining. Please check however with your microscope specialist as well, whether this is an option for you. Excited at 543nm: DyLight® 550 : max excitation at 562nm, max emission at 576 (please see link below for more info on the spectrum) -goat anti rabbit IgG H+L, preabsorbed: ab96900 Click here (or use the following: https://www.abcam.com/index.html?datasheet=96900). Cy3.5: max excitation at 576nm, max emission at 589 -goat anti rabbit IgG H+L, preabsorbed: ab97076 Click here (or use the following: https://www.abcam.com/index.html?datasheet=97076). -goat anti guinea pig IgG H+L, preabsorbed: ab6966 Click here (or use the following: https://www.abcam.com/index.html?datasheet=6966). Texas Red: max excitiation at 595, max emission at 613 -donkey anti rabbit IgG H+L, preabsorbed: ab7081 Click here (or use the following: https://www.abcam.com/index.html?datasheet=7081). TRITC: max excitiation at 557, max emission at 576 -goat anti guinea pig IgG H+L, preabsorbed: ab7136 Click here (or use the following: https://www.abcam.com/index.html?datasheet=7136). -donkey anti rabbit IgG H+L, preabsorbed: ab7080 Click here (or use the following: https://www.abcam.com/index.html?datasheet=7080). Excited also 543nm: Cy3: max excitiation at 548, max emission at 561 -goat anti rabbit IgG H+L, preabsorbed: ab97075 Click here (or use the following: https://www.abcam.com/index.html?datasheet=97075). DyLight594: max excitiation at 591 max emission at 616 -we do have the anti guinea pig antibody in this conjugation, unfortunately not preabsorved. It would therefore need to be tested first. It is the ab96961. We might can provide you with a testing offer if you would be interested in this option. -donkey anti rabbit IgG H+L, preabsorbed: ab96921 Click here (or use the following: https://www.abcam.com/index.html?datasheet=96921). As an alternative to an already conjugated antibody, I can also recommend to use either a biotynilated antibody or an unconjugated antibody. In those cases, you would either need an avidin/streptavidin in the right color or a correct conjugation kits (e.g. PerCP, tandem dyes). For the conjugation kits, I can recommend our easylink conjugation kits, very fast and easy to use: www.abcam.com/easylink Please do not hesitate to contact can we help or advise you further. We do have also a poster "guide to fluorochromes", which I can recommend to consult or to download: https://docs.abcam.com/pdf/immunology/fluorochrome_guide.pdf Alternatively, we do also have a poster on the DyLight antibodies. https://docs.abcam.com/pdf/general/DyLight_flyer.pdf I hope this information is helpful. Many season greetings!

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