Goat Anti-Guinea pig IgG H&L (HRP) preadsorbed (ab7139)

Submit an Abreview Q&A (2)Specific References (1)

Overview

  • Product name
    Goat Anti-Guinea pig IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Guinea pig
  • Tested applications
    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details
  • Immunogen

    guinea pig IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.60
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using guinea pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
  • Application notes
    suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency

    • References

    This product has been referenced in:
    • Heijde M & Ulm R Reversion of the Arabidopsis UV-B photoreceptor UVR8 to the homodimeric ground state. Proc Natl Acad Sci U S A 110:1113-8 (2013). IP ; Guinea pig . Read more (PubMed: 23277547) »

    See 1 Publication for this product

    Publishing research using ab7139? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    I`am about to do some western blotting. I was wondering what the differences are between ab6908 and ab7139. I noticed in the description that ab7139 is pre-adsorbed with HRP. How does that affect the antibody? Is there any cost difference between ...

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    Thank you for your enquiry. I apologise for the delay in responding to your email. There is little difference between these two antibodies. Ab6908 is raised against a full length purified guinea pig IgG and is protein G purified Ab7139 is r...

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    I'm using this product as a secondary antibody and, as such, only need to use a very tiny amount (1:50,000 dilution). What can I dilute this antibody in for long term storage at -20C? When I use Pierce antibodies, I dilute them 1:5 in Pierce's SuperF...

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    Thank you for your enquiry. This antibody is provided in PBS and therefore I would recommend that if you wish to dilute the antibody prior to storage , then this should be done in PBS at a dilution of 1:5 or 1:10. It may also be useful to make this...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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