Goat Anti-Guinea pig IgG H&L (HRP) preadsorbed (ab7139)

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Overview

  • Product name
    Goat Anti-Guinea pig IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Guinea pig
  • Tested applications
    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details
  • Immunogen

    guinea pig IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.60
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using guinea pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
  • Application notes
    suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency

    • References

    This product has been referenced in:
    • Heijde M & Ulm R Reversion of the Arabidopsis UV-B photoreceptor UVR8 to the homodimeric ground state. Proc Natl Acad Sci U S A 110:1113-8 (2013). IP ; Guinea pig . Read more (PubMed: 23277547) »
    See 1 Publication for this product

    Publishing research using ab7139? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I`am about to do some western blotting. I was wondering what the differences are between ab6908 and ab7139. I noticed in the description that ab7139 is pre-adsorbed with HRP. How does that affect the antibody? Is there any cost difference between the two?

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    Answer

    Thank you for your enquiry. I apologise for the delay in responding to your email. There is little difference between these two antibodies. Ab6908 is raised against a full length purified guinea pig IgG and is protein G purified Ab7139 is raised against whole guinea pig IgG. You are correct, this has been preadsorbed against serum proteins from Bovine Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat, and Sheep. This reduces the chance of the antibody cross-reacting to other proteins in these species. I hope this information is useful. In order to obtain more information on prices and availability in Japan, I suggest you contact our Japanese distributor, CosmoBio. They can be contacted using the following email address: tsawamoto@cosmobio.co.jp.

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    Question

    I'm using this product as a secondary antibody and, as such, only need to use a very tiny amount (1:50,000 dilution). What can I dilute this antibody in for long term storage at -20C? When I use Pierce antibodies, I dilute them 1:5 in Pierce's SuperFreeze and aliquot.

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    Answer

    Thank you for your enquiry. This antibody is provided in PBS and therefore I would recommend that if you wish to dilute the antibody prior to storage , then this should be done in PBS at a dilution of 1:5 or 1:10. It may also be useful to make this up as 50% glycerol. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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