Overview

  • Product name
    Goat Anti-Guinea pig IgG H&L (Texas Red ®)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Guinea pig
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Immunogen

    Full length native Guinea Pig IgG (purified).

  • Conjugation
    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using guinea pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Conjugation notes
    Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
IHC-Fr
Immunomicroscopy
Flow Cyt
ELISA
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Images

    • Immunohistochemical analysis of mouse pituitary tissue. Tissue was stained with a guinea pig primary antibody before staining with secondary antibody (ab6906 at 1/300 dilution) for 60 minutes at room temperature.

    References

    This product has been referenced in:
    • Ilie I  et al. Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway. Int J Nanomedicine 8:3345-53 (2013). ICC, Flow Cyt . Read more (PubMed: 24039418) »
    • Arriola DJ  et al. FOXO1 Transcription Factor Inhibits Luteinizing Hormone ß Gene Expression in Pituitary Gonadotrope Cells. J Biol Chem 287:33424-35 (2012). IHC-P . Read more (PubMed: 22865884) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    I have arranged a free replacement vial of ab7137 to be sent to you on order 161903 today. I hope you will be satisfied with this product.

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    Answer

    I'm very sorry to hear you are having problems with ab6906. The fact that a no primary test gave you high background is very concerning and it certainly suggests the vial you received was damaged during shipping or storage. I looked at the tracking information of your order and it seemed that the package was delayed as fedex have recorded "Customer not available or business closed" and delivered the parcel at another address first. Can I please verify that you wash in PBS several times in between steps to remove the paraformaldehyde and primary antibody? Can you please tell if if you would like a refund or a replacement? I look forward to hearing from you,

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    Question
    Answer

    Thank you for your enquiry. This antibody has not been preabsorbed against other species IgG. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Answer

    The Texas Red we use for the conjugation is Texas Red® sulfonyl chloride. The active functional group of the dye reacts with a primary amine of the antibody and forms a covalent bond. If the secondary conjugation is targeting the amine or carboxyl or sulfhydral groups, I am not surprised that it only wants to attach to the antibody. I hope this information helps. If you have any additional questions, please contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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