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    goat-human-iga-alpha-chain-dylight-650-ab96998.pdf

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Immunology Immunoglobulins Heavy Chain IgA
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Goat Anti-Human IgA alpha chain (DyLight® 650) (ab96998)

  • Datasheet
  • SDS
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  • Goat Anti-Human IgA alpha chain (DyLight® 650) (ab96998)
  • Datasheet
  • References (1)
  • Protocols

Overview

  • Product name

    Goat Anti-Human IgA alpha chain (DyLight® 650)
    See all IgA secondary antibodies
  • Host species

    Goat
  • Target species

    Human
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Immunogen

    Other Immunogen Type corresponding to Human IgA.

  • Conjugation

    DyLight® 650. Ex: 654nm, Em: 673nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    DyLight® 650 replaces DyLight® 649.
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgA
    • Secondary antibodies
    • anti-Human
    • IgA
    • Fluorophore
    • DyLight® 650

Applications

Our Abpromise guarantee covers the use of ab96998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.

DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.

ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Goat Anti-Human IgA alpha chain (DyLight® 650) (ab96998)
    Goat Anti-Human IgA alpha chain (DyLight® 650) (ab96998)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Datasheets and documents

    • Datasheet
    • SDS
  • References

    This product has been referenced in:

    • Planer JD  et al. Development of the gut microbiota and mucosal IgA responses in twins and gnotobiotic mice. Nature 534:263-6 (2016). Read more (PubMed: 27279225) »
    See 1 Publication for this product

    Publishing research using ab96998? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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