Goat Anti-Human IgA alpha chain (DyLight® 650) (ab96998)

Submit a review Submit a question References (1)

Key features and details

  • Goat Anti-Human IgA alpha chain (DyLight® 650)
  • Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, Flow Cyt

Overview

  • Product name

    Goat Anti-Human IgA alpha chain (DyLight® 650)
    See all IgA secondary antibodies

  • Host species

    Goat

  • Target species

    Human

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details

  • Immunogen

    Other Immunogen Type corresponding to Human IgA.

  • Conjugation

    DyLight® 650. Ex: 654nm, Em: 673nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    DyLight® 650 replaces DyLight® 649.

  • Research areas

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab96998 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P 1/50 - 1/500.

    DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.
    ICC/IF 1/50 - 1/500.
    Flow Cyt 1/50 - 1/200.

    Images

    • Goat Anti-Human IgA alpha chain (DyLight® 650) (ab96998)
      Goat Anti-Human IgA alpha chain (DyLight® 650) (ab96998)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab96998? Please let us know so that we can cite the reference in this datasheet.

    ab96998 has been referenced in 1 publication.

    • Planer JD  et al. Development of the gut microbiota and mucosal IgA responses in twins and gnotobiotic mice. Nature 534:263-6 (2016). PubMed: 27279225

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab96998.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com