Goat Anti-Human IgA alpha chain (DyLight® 650) (ab96998)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Goat Anti-Human IgA alpha chain (DyLight® 650)
See all IgA secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Immunogen
Other Immunogen Type corresponding to Human IgA.
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Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 650 replaces DyLight® 649. -
Research areas
Applications
Our Abpromise guarantee covers the use of ab96998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
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| ICC/IF | 1/50 - 1/500. | |
| Flow Cyt | 1/50 - 1/200. |
Images
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Goat Anti-Human IgA alpha chain (DyLight® 650) (ab96998)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
References
This product has been referenced in:
- Planer JD et al. Development of the gut microbiota and mucosal IgA responses in twins and gnotobiotic mice. Nature 534:263-6 (2016). Read more (PubMed: 27279225) »
