Key features and details
- Goat Anti-Human IgA H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Suitable for: IHC-P
Product nameGoat Anti-Human IgA H&L (FITC)
See all IgA secondary antibodies
SpecificityAntibodies were raised in goat to human IgA. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgA is depostited. No cross reactivity with albumine fractions of human serum. No cross reactivity with albumin fractions of animal species.
Tested applicationsSuitable for: IHC-Pmore details
ConjugationFITC. Ex: 493nm, Em: 528nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesFrom the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
Conjugation notesThe purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgG.
Our Abpromise guarantee covers the use of ab8500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab8500 has not yet been referenced specifically in any publications.