Overview

  • Product name

    Goat Anti-Human IgA H&L (FITC)
    See all IgA secondary antibodies
  • Host species

    Goat
  • Target species

    Human
  • Specificity

    Antibodies were raised in goat to human IgA. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgA is depostited. No cross reactivity with albumine fractions of human serum. No cross reactivity with albumin fractions of animal species.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.50
    Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    From the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
  • Conjugation notes

    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • Clonality

    Polyclonal
  • General notes

    F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgG.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/40.

References

ab8500 has not yet been referenced specifically in any publications.

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