• Product name

    Goat Anti-Human IgE epsilon chain
    See all IgE secondary antibodies
  • Host species

  • Target species

  • Specificity

    The affinity purified anti IgE reacts only with IgE ( several different IgE kappa and lambda myeloma proteins) by IEP and gel diffusion techniques.
  • Tested applications

    Suitable for: ELISA, Immunodiffusion, Immunoelectrophoresis, WBmore details
  • Immunogen

    Goats were immunized with a mixture of several highly purified human IgE myeloma proteins in Freund’s adjuvant


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 0.194% Methyl mannoside, 0.812% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Serum was collected and passed through several non-IgE containing immunoaffinity supports to make the preparation delta chain specific. The anti delta was immunoaffinity purified off an immunoabsorbant containing a different IgE than was used for immunization thus minimizing idiotypic influences.
  • Clonality

  • Research areas


Our Abpromise guarantee covers the use of ab9159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Immunodiffusion Use at an assay dependent concentration.
Immunoelectrophoresis Use at an assay dependent concentration.
WB Use at an assay dependent concentration.


This product has been referenced in:

  • Vanshylla K  et al. Grb2 and GRAP connect the B cell antigen receptor to Erk MAP kinase activation in human B cells. Sci Rep 8:4244 (2018). Read more (PubMed: 29523808) »
  • Olivier CE  et al. In search of a tolerance-induction strategy for cow's milk allergies: significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization. Clinics (Sao Paulo) 67:1171-9 (2012). Read more (PubMed: 23070344) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


Thank you for your patience. ab9159 is a Goat IgG antibody. The specific isotype has not been determined. We can offer the antibody without azide, but the minimum order is 5 mg. Unfortunately we do not have any other anti-human IgE abs that are an fab fragment, non-rabbit host and immunogen affinity purified. Please let me know if there is any other information needed.  

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Thank you for contacting us and for your interest in our products. I am sorry to confirm that as far as we are aware, ab9159 Goat polyclonal Secondary Antibody to Human IgE ] has never been tested with samples from cynomolgus monkey. All tested and species cross-reactivity information covered by our guarantee is stated on our datasheets, and these are updated as soon as any new information is brought to our attention. We like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.  

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Thank you for your enquiry. This secondary antibody has been tested for application in Western blotting and I would recommend starting with a dilution of 1:1000 and optimize based upon results. Please note that our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If you have any additional questions, please contact us again.

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Thank you for your enquiry. We currently do not have a Human IgE polyclonal antibody that is conjugated to HRP or AP. We do have a monoclonal: ab7426 - Mouse monoclonal (GE-1) to Human IgE (alkaline phosphatase). If you have any more questions, please contact us again.

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