Goat Anti-Human IgG (Fab')2 (HRP) (ab87422)
Key features and details
- Goat Anti-Human IgG (Fab')2 (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, WB
Overview
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Product name
Goat Anti-Human IgG (Fab')2 (HRP)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
ab87422 is specific for the Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity. -
Tested applications
Suitable for: ELISA, WBmore details -
Immunogen
Other Immunogen Type corresponding to IgG. Human serum.
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
This antibody has been cross adsorbed against the Fc domain and will react with less than 1 % of the Fc domain of human IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/1000 - 1/10000.
|
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| WB |
1/1000 - 1/10000.
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| Notes |
|---|
|
ELISA
1/1000 - 1/10000. |
|
WB
1/1000 - 1/10000. |
Images
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ELISA - Goat Anti-Human IgG (Fab')2 (HRP) (ab87422)Marino et al PLoS One. 2017 May 18;12(5):e0177810. doi: 10.1371/journal.pone.0177810. eCollection 2017. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Characterisation of virus activity and functional expression of monoclonal antibodies.
(Panel D) IgG1 antibody expression, assessed by anti-IgG1 ELISA, in cellular supernatants of carcinoma cell lines infected for 24-96hrs with NG-135 at 1ppc.
Media was removed from infected or control cells, clarified by centrifugation and diluted 1 in 2 in 3% BSA/PBS for storage at -20°C. For quantification of human IgG1, 96 well plates were coated overnight, 4°C, with mouse monoclonal anti-human IgG1 Fc antibody (ab1927, Abcam) at a 1:1000 dilution in carbonate/bicarbonate buffer. Plates were blocked in 3% BSA/PBS, washed and samples and standards added to the plate for 1 hr and RT. Secondary detection was carried out using a HRP conjugated goat anti-human IgG Fab (ab87422, Abcam) incubated for 1 hr, RT.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab87422 has been referenced in 3 publications.
- Sasso E et al. Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies. MAbs 10:1060-1072 (2018). PubMed: 29995563
- Marino N et al. Development of a versatile oncolytic virus platform for local intra-tumoural expression of therapeutic transgenes. PLoS One 12:e0177810 (2017). PubMed: 28542292
- Andrabi SA et al. Poly(ADP-ribose) polymerase-dependent energy depletion occurs through inhibition of glycolysis. Proc Natl Acad Sci U S A 111:10209-14 (2014). PubMed: 24987120