Key features and details
- Goat Anti-Human IgG (Fab')2 (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, WB
Product nameGoat Anti-Human IgG (Fab')2 (HRP)
See all IgG secondary antibodies
Specificityab87422 is specific for the Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity.
Tested applicationsSuitable for: ELISA, WBmore details
Other Immunogen Type corresponding to IgG. Human serum.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody has been cross adsorbed against the Fc domain and will react with less than 1 % of the Fc domain of human IgG.
Our Abpromise guarantee covers the use of ab87422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/1000 - 1/10000.|
|WB||1/1000 - 1/10000.|
Characterisation of virus activity and functional expression of monoclonal antibodies.
(Panel D) IgG1 antibody expression, assessed by anti-IgG1 ELISA, in cellular supernatants of carcinoma cell lines infected for 24-96hrs with NG-135 at 1ppc.
Media was removed from infected or control cells, clarified by centrifugation and diluted 1 in 2 in 3% BSA/PBS for storage at -20°C. For quantification of human IgG1, 96 well plates were coated overnight, 4°C, with mouse monoclonal anti-human IgG1 Fc antibody (ab1927, Abcam) at a 1:1000 dilution in carbonate/bicarbonate buffer. Plates were blocked in 3% BSA/PBS, washed and samples and standards added to the plate for 1 hr and RT. Secondary detection was carried out using a HRP conjugated goat anti-human IgG Fab (ab87422, Abcam) incubated for 1 hr, RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab87422 has been referenced in 3 publications.
- Sasso E et al. Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies. MAbs 10:1060-1072 (2018). PubMed: 29995563
- Marino N et al. Development of a versatile oncolytic virus platform for local intra-tumoural expression of therapeutic transgenes. PLoS One 12:e0177810 (2017). PubMed: 28542292
- Andrabi SA et al. Poly(ADP-ribose) polymerase-dependent energy depletion occurs through inhibition of glycolysis. Proc Natl Acad Sci U S A 111:10209-14 (2014). PubMed: 24987120