Product nameGoat Anti-Human IgG Fc
See all IgG secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
Tested applicationsSuitable for: IHC-P, ELISA, WB, ICCmore details
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Our Abpromise guarantee covers the use of ab97221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/2000.|
|ELISA||1/100 - 1/500. for coating; 1/1000 - 1/30000 as primary|
|WB||1/1000 - 1/10000. Colorimetric; 1/1000 - 1/30000 Chemiluminescent|
|ICC||Use at an assay dependent dilution.|
This product has been referenced in:
- Zou Y & Li L Identification of six serum antigens and autoantibodies for the detection of early stage epithelial ovarian carcinoma by bioinformatics analysis and liquid chip analysis. Oncol Lett 16:3231-3240 (2018). Read more (PubMed: 30127919) »
- Salem AF et al. Reduction of Circulating Cancer Cells and Metastases in Breast-Cancer Models by a Potent EphA2-Agonistic Peptide-Drug Conjugate. J Med Chem 61:2052-2061 (2018). Read more (PubMed: 29470068) »