Key features and details
- Goat Anti-Human IgG Fc (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, WB, IHC-P, ELISA
Product nameGoat Anti-Human IgG Fc (Alkaline Phosphatase)
See all IgG secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
Tested applicationsSuitable for: ICC, WB, IHC-P, ELISAmore details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.10
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.1% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Conjugation notesMolar enzyme: antibody protein ratio is 1:1
Our Abpromise guarantee covers the use of ab97222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|WB||1/5000 - 1/50000. (Chemiluminescent)|
|IHC-P||1/100 - 1/1000.|
|ELISA||1/5000 - 1/50000. (Primary)|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97222 has not yet been referenced specifically in any publications.