Goat Anti-Human IgG Fc (Biotin) (ab97223)
Key features and details
- Goat Anti-Human IgG Fc (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
Related conjugates and formulations
Overview
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Product name
Goat Anti-Human IgG Fc (Biotin)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. -
Tested applications
Suitable for: IHC-P, ICC/IF, ELISA, WBmore details -
Immunogen
Other Immunogen Type corresponding to Human IgG.
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/250 - 1/2500.
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| ICC/IF |
1/100 - 1/500.
|
|
| ELISA |
1/10000 - 1/200000. (Primary)
|
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| WB |
1/10000 - 1/200000. (Chemiluminescent)
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| Notes |
|---|
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IHC-P
1/250 - 1/2500. |
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ICC/IF
1/100 - 1/500. |
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ELISA
1/10000 - 1/200000. (Primary) |
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WB
1/10000 - 1/200000. (Chemiluminescent) |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab97223 has been referenced in 6 publications.
- Bachhuber F et al. Diagnostic biomarkers in tear fluid: from sampling to preanalytical processing. Sci Rep 11:10064 (2021). PubMed: 33980989
- Simao M et al. cAMP Bursts Control T Cell Directionality by Actomyosin Cytoskeleton Remodeling. Front Cell Dev Biol 9:633099 (2021). PubMed: 34095108
- Ko SY et al. Cancer-derived small extracellular vesicles promote angiogenesis by heparin-bound, bevacizumab-insensitive VEGF, independent of vesicle uptake. Commun Biol 2:386 (2019). PubMed: 31646189
- Altman JB et al. Optimized protocol for detection of native, full-length HIV-1 envelope on the surface of transfected cells. Health Sci Rep 1:e74 (2018). PubMed: 30623097
- Khetarpal SA et al. A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels. Nat Med 23:1086-1094 (2017). PubMed: 28825717
- Mortensen AH et al. Deletion of OTX2 in neural ectoderm delays anterior pituitary development. Hum Mol Genet 24:939-53 (2015). PubMed: 25315894