Goat Anti-Human IgG Fc (Biotin) (ab97223)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameGoat Anti-Human IgG Fc (Biotin)
See all IgG secondary antibodies -
Host speciesGoat
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Target speciesHuman
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SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
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Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, WBmore details
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Immunogen
Other Immunogen Type corresponding to Human IgG.
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ConjugationBiotin
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab97223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/250 - 1/2500. | |
| ICC/IF | 1/100 - 1/500. | |
| ELISA | 1/10000 - 1/200000. (Primary) | |
| WB | 1/10000 - 1/200000. (Chemiluminescent) |
References
This product has been referenced in:
- Mortensen AH et al. Deletion of OTX2 in neural ectoderm delays anterior pituitary development. Hum Mol Genet 24:939-53 (2015). Read more (PubMed: 25315894) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"