Goat Anti-Human IgG Fc (Biotin) (ab97223)
- Datasheet
- References (3)
- Protocols
Overview
-
Product nameGoat Anti-Human IgG Fc (Biotin)
See all IgG secondary antibodies -
Host speciesGoat
-
Target speciesHuman
-
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
-
Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, WBmore details
-
Immunogen
Other Immunogen Type corresponding to Human IgG.
-
ConjugationBiotin
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Applications
Our Abpromise guarantee covers the use of ab97223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/250 - 1/2500. | |
| ICC/IF | 1/100 - 1/500. | |
| ELISA | 1/10000 - 1/200000. (Primary) | |
| WB | 1/10000 - 1/200000. (Chemiluminescent) |
References
This product has been referenced in:
- Altman JB et al. Optimized protocol for detection of native, full-length HIV-1 envelope on the surface of transfected cells. Health Sci Rep 1:e74 (2018). Read more (PubMed: 30623097) »
- Khetarpal SA et al. A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels. Nat Med 23:1086-1094 (2017). Read more (PubMed: 28825717) »
