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  1. Link

    goat-human-igg-fc-biotin-ab97223.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Human IgG Fc (Biotin) (ab97223)

  • Datasheet
  • SDS
Submit a review Submit a question References (6)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Goat Anti-Human IgG Fc (Biotin)
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, ELISA, WB

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Overview

  • Product name

    Goat Anti-Human IgG Fc (Biotin)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Human
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Immunogen

    Other Immunogen Type corresponding to Human IgG.

  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Human
    • IgG
    • Biotin

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/250 - 1/2500.
ICC/IF
1/100 - 1/500.
ELISA
1/10000 - 1/200000. (Primary)
WB
1/10000 - 1/200000. (Chemiluminescent)
Notes
IHC-P
1/250 - 1/2500.
ICC/IF
1/100 - 1/500.
ELISA
1/10000 - 1/200000. (Primary)
WB
1/10000 - 1/200000. (Chemiluminescent)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (6)

Publishing research using ab97223? Please let us know so that we can cite the reference in this datasheet.

ab97223 has been referenced in 6 publications.

  • Bachhuber F  et al. Diagnostic biomarkers in tear fluid: from sampling to preanalytical processing. Sci Rep 11:10064 (2021). PubMed: 33980989
  • Simao M  et al. cAMP Bursts Control T Cell Directionality by Actomyosin Cytoskeleton Remodeling. Front Cell Dev Biol 9:633099 (2021). PubMed: 34095108
  • Ko SY  et al. Cancer-derived small extracellular vesicles promote angiogenesis by heparin-bound, bevacizumab-insensitive VEGF, independent of vesicle uptake. Commun Biol 2:386 (2019). PubMed: 31646189
  • Altman JB  et al. Optimized protocol for detection of native, full-length HIV-1 envelope on the surface of transfected cells. Health Sci Rep 1:e74 (2018). PubMed: 30623097
  • Khetarpal SA  et al. A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels. Nat Med 23:1086-1094 (2017). PubMed: 28825717
  • Mortensen AH  et al. Deletion of OTX2 in neural ectoderm delays anterior pituitary development. Hum Mol Genet 24:939-53 (2015). PubMed: 25315894

Customer reviews and Q&As

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