Overview

  • Product name

    Goat Anti-Human IgG Fc (DyLight® 550)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Human
  • Specificity

    The antibody is specific to Fc region of human IgG. The Dylight conjugate is conjugated at Fc region of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Conjugation

    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    DyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.

DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.

ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

ab97004 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for contacting us. The antibody is indeed specific to the Fc region of human IgG and the Dylight conjugate is conjugated to the Fc region of antibody. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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