Key features and details
- Goat Anti-Human IgG Fc (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Product nameGoat Anti-Human IgG Fc (FITC)
See all IgG secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
Other Immunogen Type corresponding to Human IgG.
ConjugationFITC. Ex: 493nm, Em: 528nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
Conjugation notesF/P ratio is 5
Our Abpromise guarantee covers the use of ab97224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/500.|
|ICC/IF||1/50 - 1/500.|
|Flow Cyt||1/50 - 1/200.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97224 has been referenced in 4 publications.
- Liu W et al. Structural Basis of CD160:HVEM Recognition. Structure 27:1286-1295.e4 (2019). PubMed: 31230945
- Hamorsky KT et al. Engineering of a Lectibody Targeting High-Mannose-Type Glycans of the HIV Envelope. Mol Ther 27:2038-2052 (2019). PubMed: 31471224
- Lee CH et al. IgG Fc domains that bind C1q but not effector Fc? receptors delineate the importance of complement-mediated effector functions. Nat Immunol 18:889-898 (2017). PubMed: 28604720
- Li JQ et al. Generation of induced pluripotent stem cells using skin fibroblasts from patients with myocardial infarction under feeder-free conditions. Mol Med Rep 9:837-42 (2014). PubMed: 24398533