Goat Anti-Human IgG Fc (FITC) (ab97224)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Goat Anti-Human IgG Fc (FITC)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Immunogen
Other Immunogen Type corresponding to Human IgG.
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC. -
Conjugation notes
F/P ratio is 5 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
Our Abpromise guarantee covers the use of ab97224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/500. | |
| ICC/IF | 1/50 - 1/500. | |
| Flow Cyt | 1/50 - 1/200. |
Datasheets and documents
References
This product has been referenced in:
- Lee CH et al. IgG Fc domains that bind C1q but not effector Fc? receptors delineate the importance of complement-mediated effector functions. Nat Immunol 18:889-898 (2017). Read more (PubMed: 28604720) »
