Overview

  • Product name

    Goat Anti-Human IgG Fc (FITC)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Human
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Immunogen

    Other Immunogen Type corresponding to Human IgG.

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Conjugation notes

    F/P ratio is 5
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

References

This product has been referenced in:

  • Lee CH  et al. IgG Fc domains that bind C1q but not effector Fc? receptors delineate the importance of complement-mediated effector functions. Nat Immunol 18:889-898 (2017). Read more (PubMed: 28604720) »
See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Immunocytochemistry
This antibody has been stored at 4C for several years and I was very surprised that it still works very well in staining live cells.

Abcam Note: While we are excited to hear that the antibody has performed well after all this time, we guarantee the performance of all of our products for one year from their date of receipt.

Abcam user community

Verified customer

Submitted Oct 31 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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