Goat Anti-Human IgG Fc (FITC) (ab97224)
Key features and details
- Goat Anti-Human IgG Fc (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Overview
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Product name
Goat Anti-Human IgG Fc (FITC)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Immunogen
Other Immunogen Type corresponding to Human IgG.
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC. -
Conjugation notes
F/P ratio is 5 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
Applications
Our Abpromise guarantee covers the use of ab97224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/500. | |
| ICC/IF | 1/50 - 1/500. | |
| Flow Cyt | 1/50 - 1/200. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (4)
ab97224 has been referenced in 4 publications.
- Liu W et al. Structural Basis of CD160:HVEM Recognition. Structure 27:1286-1295.e4 (2019). PubMed: 31230945
- Hamorsky KT et al. Engineering of a Lectibody Targeting High-Mannose-Type Glycans of the HIV Envelope. Mol Ther 27:2038-2052 (2019). PubMed: 31471224
- Lee CH et al. IgG Fc domains that bind C1q but not effector Fc? receptors delineate the importance of complement-mediated effector functions. Nat Immunol 18:889-898 (2017). PubMed: 28604720
- Li JQ et al. Generation of induced pluripotent stem cells using skin fibroblasts from patients with myocardial infarction under feeder-free conditions. Mol Med Rep 9:837-42 (2014). PubMed: 24398533
