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    goat-human-igg-fc-fitc-ab97224.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Human IgG Fc (FITC) (ab97224)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (4)

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Goat Anti-Human IgG Fc (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, Flow Cyt

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Overview

  • Product name

    Goat Anti-Human IgG Fc (FITC)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Human
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Immunogen

    Other Immunogen Type corresponding to Human IgG.

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Human
    • IgG
    • Fluorophore
    • FITC

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab97224 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P 1/50 - 1/500.
    ICC/IF 1/50 - 1/500.
    Flow Cyt 1/50 - 1/200.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab97224? Please let us know so that we can cite the reference in this datasheet.

    ab97224 has been referenced in 4 publications.

    • Liu W  et al. Structural Basis of CD160:HVEM Recognition. Structure 27:1286-1295.e4 (2019). PubMed: 31230945
    • Hamorsky KT  et al. Engineering of a Lectibody Targeting High-Mannose-Type Glycans of the HIV Envelope. Mol Ther 27:2038-2052 (2019). PubMed: 31471224
    • Lee CH  et al. IgG Fc domains that bind C1q but not effector Fc? receptors delineate the importance of complement-mediated effector functions. Nat Immunol 18:889-898 (2017). PubMed: 28604720
    • Li JQ  et al. Generation of induced pluripotent stem cells using skin fibroblasts from patients with myocardial infarction under feeder-free conditions. Mol Med Rep 9:837-42 (2014). PubMed: 24398533

    Customer reviews and Q&As

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    works well with humanized primary antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    This antibody has been stored at 4C for several years and I was very surprised that it still works very well in staining live cells.

    Abcam Note: While we are excited to hear that the antibody has performed well after all this time, we guarantee the performance of all of our products for one year from their date of receipt.

    Abcam user community

    Verified customer

    Submitted Oct 31 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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