Overview

  • Product name

    Goat Anti-Human IgG Fc (HRP)
    See all IgG secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Human IgG - Fc (HRP)
  • Host species

    Goat
  • Target species

    Human
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Immunogen

    Other Immunogen Type corresponding to Human IgG.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 0.2% BSA, PBS, 0.00062% 5-chloro-2-methyl-4-isothiazolin-3-one, 0.00062% 2-methyl-4-isothiazolin-3-one
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes

    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary)
WB 1/2000 - 1/25000. Colormetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000

References

This product has been referenced in:

  • Stockdale L  et al. HIV, HCMV and mycobacterial antibody levels: a cross-sectional study in a rural Ugandan cohort. Trop Med Int Health 24:247-257 (2019). Read more (PubMed: 30506614) »
  • Chudzian J  et al. Specific Antibody Fragment Ligand Traps Blocking FGF1 Activity. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 30134556) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

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Answer

I'm happy to help! Please contact me again if you need anything further.

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Answer

Thanks for your enquiry. Attached please find the CoC.

I hope this is helpful. Please contact me again if you have any further questions.

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Answer

Thank you for contacting us.

The datasheet of these antibodies say "Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles"

This mean after receiving the antibody you can keep antibody at 4C for 1-2 weeks when using in your experiments. After you finish your experiment we recomendaliquoting the antibody 5-10 ul in vialsand storing at -20C. We suggest this to avoidthe extra freeze thawing steps, every time you use the antibody.

If you have already stored it at -20C, this is absolutely fine!

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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