Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)

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Overview

  • Product name

    Goat Anti-Human IgG Fc (HRP) preadsorbed
    See all IgG secondary antibodies

  • Description

    Goat polyclonal Secondary Antibody to Human IgG - Fc (HRP), pre-adsorbed

  • Host species

    Goat

  • Target species

    Human

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, Sheep more details

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituents: PBS, 0.2% BSA, 0.00063% 2-methyl-4-isothiazolin-3-one, 0.00063% 5-chloro-2-methyl-4-isothiazolin-3-one
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000).

References

This product has been referenced in:

  • Yang H  et al. A study of anti-gliadin antibodies in first-episode patients with schizophrenia among a Chinese population. Psychiatry Res 272:454-457 (2019). Read more (PubMed: 30611964) »
  • Wang C  et al. Enhanced expression of ten-eleven translocation 1 reverses gemcitabine resistance in cholangiocarcinoma accompanied by a reduction in P-glycoprotein expression. Cancer Med 8:990-1003 (2019). Read more (PubMed: 30784212) »
See all 5 Publications for this product

Publishing research using ab98624? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Testing of Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) in Dot blot

 Excellent
Abreviews
abreview image
Application
Dot blot
Human IgG1 was used as standard series for Dot blot. 2µl sample (in duplicates) or different dilutions of human IgG1 were blotted on a nitrocellulose membrane (0.45µm, Ultra Cruz). The membrane was incubated for 1h at RT with anti-human IgG Fc antibody (HRP) preadsorbed (ab98624) in TBS-T. The blot was developed using ECL substrate and chemiluminescence detected.


Result: Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) is suitable for Dot blot

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Submitted Sep 21 2017

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