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Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)

Reviews (2) Submit a question References (11)

Key features and details

  • Goat Anti-Human IgG Fc (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

Overview

  • Product name

    Goat Anti-Human IgG Fc (HRP) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Human

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, Sheep more details

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Constituents: PBS, 0.2% BSA, 0.00063% 2-methyl-4-isothiazolin-3-one, 0.00063% 5-chloro-2-methyl-4-isothiazolin-3-one
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab98624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (Primary).
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000).
Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (Primary).
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (11)

Publishing research using ab98624? Please let us know so that we can cite the reference in this datasheet.

ab98624 has been referenced in 11 publications.

  • Lenders M  et al. Generation and Characterization of a Polyclonal Human Reference Antibody to Measure Anti-Drug Antibody Titers in Patients with Fabry Disease. Int J Mol Sci 22:N/A (2021). PubMed: 33800950
  • Ebihara T  et al. Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System. Mol Biotechnol 63:1223-1234 (2021). PubMed: 34304364
  • Yu X  et al. Isotype Switching Converts Anti-CD40 Antagonism to Agonism to Elicit Potent Antitumor Activity. Cancer Cell 37:850-866.e7 (2020). PubMed: 32442402
  • Yi Y  et al. Clinical significance of herpes virus entry mediator expression in hepatitis B virus-related hepatocellular carcinoma. Oncol Lett 20:19 (2020). PubMed: 32774492
  • Li C  et al. Anti-TSNARE1 IgG plasma levels differ by sex in patients with schizophrenia in a Chinese population. FEBS Open Bio 9:1705-1712 (2019). PubMed: 31336035
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

ELISA-based measurement of anti-drug antibodies from human sera

 Excellent
Abreviews
abreview image
Application
Sandwich ELISA
96-well plates were coated with 100 ng agalsidase-alfa (Takeda) per well over night at 4 °C and washed three times with PBS. For negative controls, 100 ng BSA per well was used. After blocking with 2% BSA/PBS for 1 h at room temperature, wells were washed once with PBS. To detect anti-agalsidase-alfa antibodies from patients’ sera, serial dilutions from individual raw sera were loaded into the wells and incubated for 2 h at room temperature. After five washing steps with 0.1% Tween-20/PBS, anti-hIgG antibodies conjugated with HRP (ab98624, Abcam working concentration: 20 ng/mL) were applied and incubated for 1 h at room temperature. Wells were washed again five times with 0.1% Tween-20/PBS. For IgG detection 50 µL 1-Step TMB-ELISA Substrate Solution (Thermo Fisher Scientific, Darmstadt, Germany) were added to the wells, followed by 50 µL 2 M sulfuric acid to stop the reaction after 15 to 20 min. Absorption was measured at 450 nm. A serial dilution of the anti-alpha-galactosidase A reference antibody (Lenders et al. Int. J. Mol. Sci. 2021, 22, 2680) starting with 800 pg/µL, was used as reference.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 04 2021

Testing of Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) in Dot blot

 Excellent
Abreviews
abreview image
Application
Dot blot
Human IgG1 was used as standard series for Dot blot. 2µl sample (in duplicates) or different dilutions of human IgG1 were blotted on a nitrocellulose membrane (0.45µm, Ultra Cruz). The membrane was incubated for 1h at RT with anti-human IgG Fc antibody (HRP) preadsorbed (ab98624) in TBS-T. The blot was developed using ECL substrate and chemiluminescence detected.


Result: Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) is suitable for Dot blot
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 21 2017

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