Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)

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Key features and details

  • Goat Anti-Human IgG Fc (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

Overview

  • Product name

    Goat Anti-Human IgG Fc (HRP) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Human

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, Sheep more details

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Constituents: PBS, 0.2% BSA, 0.00063% 2-methyl-4-isothiazolin-3-one, 0.00063% 5-chloro-2-methyl-4-isothiazolin-3-one
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab98624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (Primary).
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000).
Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (Primary).
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (9)

Publishing research using ab98624? Please let us know so that we can cite the reference in this datasheet.

ab98624 has been referenced in 9 publications.

  • Yu X  et al. Isotype Switching Converts Anti-CD40 Antagonism to Agonism to Elicit Potent Antitumor Activity. Cancer Cell 37:850-866.e7 (2020). PubMed: 32442402
  • Yi Y  et al. Clinical significance of herpes virus entry mediator expression in hepatitis B virus-related hepatocellular carcinoma. Oncol Lett 20:19 (2020). PubMed: 32774492
  • Li C  et al. Anti-TSNARE1 IgG plasma levels differ by sex in patients with schizophrenia in a Chinese population. FEBS Open Bio 9:1705-1712 (2019). PubMed: 31336035
  • Mitran CJ  et al. Antibodies to Cryptic Epitopes in Distant Homologues Underpin a Mechanism of Heterologous Immunity between Plasmodium vivax PvDBP and Plasmodium falciparum VAR2CSA. mBio 10:N/A (2019). PubMed: 31594821
  • Yang H  et al. A study of anti-gliadin antibodies in first-episode patients with schizophrenia among a Chinese population. Psychiatry Res 272:454-457 (2019). PubMed: 30611964
  • Wang C  et al. Enhanced expression of ten-eleven translocation 1 reverses gemcitabine resistance in cholangiocarcinoma accompanied by a reduction in P-glycoprotein expression. Cancer Med 8:990-1003 (2019). PubMed: 30784212
  • Whelan R  et al. Study of Novel Autoantibodies in Schizophrenia. Schizophr Bull 44:1341-1349 (2018). PubMed: 29373732
  • McLean RT  et al. Differential antibody responses to gliadin-derived indigestible peptides in patients with schizophrenia. Transl Psychiatry 7:e1121 (2017). ELISA ; Human . PubMed: 28485731
  • Qu F  et al. MiR-199a-3p suppresses proliferation and invasion of prostate cancer cells by targeting Smad1. Oncotarget 8:52465-52473 (2017). PubMed: 28881744

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Testing of Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) in Dot blot

 Excellent
Abreviews
abreview image
Application
Dot blot
Human IgG1 was used as standard series for Dot blot. 2µl sample (in duplicates) or different dilutions of human IgG1 were blotted on a nitrocellulose membrane (0.45µm, Ultra Cruz). The membrane was incubated for 1h at RT with anti-human IgG Fc antibody (HRP) preadsorbed (ab98624) in TBS-T. The blot was developed using ECL substrate and chemiluminescence detected.


Result: Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) is suitable for Dot blot

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Verified customer

Submitted Sep 21 2017

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