Human IgG1 was used as standard series for Dot blot. 2µl sample (in duplicates) or different dilutions of human IgG1 were blotted on a nitrocellulose membrane (0.45µm, Ultra Cruz). The membrane was incubated for 1h at RT with anti-human IgG Fc antibody (HRP) preadsorbed (ab98624) in TBS-T. The blot was developed using ECL substrate and chemiluminescence detected.
Result: Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) is suitable for Dot blot
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Submitted Sep 21 2017
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