The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/200 - 1/2000.
1/100 - 1/30000. Coating: Use 1/100-1/500 dilution; Primary: Use 1/1,000-1/30,000 dilution.
1/1000 - 1/30000. Colorimetric: Use 1/1,000-1/10,000 dilution; Chemiluminescent: Use 1/1,000-1/30,000 dilution.
This product has been referenced in:
Mathias S et al. Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy. J Biotechnol271:47-55 (2018).
Read more (PubMed: 29477386) »