Key features and details
- Goat Anti-Human IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, ELISA, WB, IHC-P
Product nameGoat Anti-Human IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
Tested applicationsSuitable for: ICC, ELISA, WB, IHC-Pmore details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Conjugation notesMolar enzyme: antibody protein ratio is 1:1
Our Abpromise guarantee covers the use of ab97162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|ELISA||1/5000 - 1/50000. (as Primary).|
|WB||1/5000 - 1/50000. (Chemiluminescent).|
|IHC-P||1/100 - 1/1000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97162 has been referenced in 1 publication.
- Yan L et al. Paraneoplastic cerebellar degeneration associated with breast cancer: A case report and review of the literature. Mol Clin Oncol 9:163-167 (2018). PubMed: 30101014