Overview

  • Product name

    Goat Anti-Human IgG H&L (Cy3 ®) preadsorbed
    See all IgG secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Human IgG - H&L (Cy3 ®), pre-adsorbed
  • Host species

    Goat
  • Target species

    Human
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, mouse, pig, rabbit and rat was detected.

     

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Horse, Mouse, Pig, Rabbit, Rat more details
  • Conjugation

    Cy3 ®. Ex: 552nm, Em: 565nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, horse, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3 ®.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/100 - 1/1000.

References

This product has been referenced in:

  • Polajnar M  et al. Expanding the host cell ubiquitylation machinery targeting cytosolic Salmonella. EMBO Rep 18:1572-1585 (2017). Read more (PubMed: 28784601) »
  • Carmona SJ  et al. Towards High-throughput Immunomics for Infectious Diseases: Use of Next-generation Peptide Microarrays for Rapid Discovery and Mapping of Antigenic Determinants. Mol Cell Proteomics 14:1871-84 (2015). Read more (PubMed: 25922409) »
See all 2 Publications for this product

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