Overview

  • Product name

    Goat Anti-Human IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Human
  • Specificity

    Antibodies were raised in goat to human IgG, IgM and IgA
  • Tested applications

    Suitable for: Flow Cytmore details
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.50
    Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Specific antibodies were absorpted by incubation with Sepharose-bound human immuno- globulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human immunoglobulins a minimum of 65% protein bound.
  • Conjugation notes

    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • Clonality

    Polyclonal
  • General notes

    F/P ratio is 4-6 mol Fluorescein per 1 mol of goat Ig. There is low background fluorescence in normal tissue in contrast to cases of auto immune desease where Ig's are depostited.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent dilution.

References

ab8503 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in FACS. All tested applications are specified on Abcam product datasheets. Please note that this antibody is not available to customers within the US due to import restrictions. US Customs are currently preventing all antibodies raised in sheep, goat, donkey, horse, pig and chicken from entering into the States unless you have a USDA import permit in place for these items. Abcam’s US office in Cambridge MA holds an import permit that covers for the majority of antibodies in our range that are derived from farmyard animals outside the USA. Unfortunately our permit does not cover this product and thus we are unable to ship it to our customers in USA. If you have any additional questions, please contact us again.

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