Goat Anti-Human IgG H&L (HRP) (ab6858)

Submit a review Q&A (4)References (22)
Dot Blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

    Key features and details

    • Goat Anti-Human IgG H&L (HRP)
    • Conjugation: HRP
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

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    Overview

    • Product name

      Goat Anti-Human IgG H&L (HRP)
      See all IgG secondary antibodies

    • Host species

      Goat

    • Target species

      Human

    • Tested applications

      Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

    • Immunogen

      human IgG whole molecule

    • Conjugation

      HRP

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      Preservative: 0.01% Gentamicin sulphate
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • Purity

      Affinity purified

    • Purification notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • Conjugation notes

      Horseradish Peroxidase (HRP)

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6858 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/1000 - 1/5000.
    Dot blot
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent dilution.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    Immunomicroscopy
    Use at an assay dependent concentration.
    WB
    1/5000 - 1/25000.
    Notes
    ICC/IF
    1/1000 - 1/5000.
    Dot blot
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent dilution.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    Immunomicroscopy
    Use at an assay dependent concentration.
    WB
    1/5000 - 1/25000.

    Images

    • Dot Blot - Goat Anti-Human IgG H&L (HRP) (ab6858)
      Dot Blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

      Dot blot analysis of human IgG using ab6858 at 1/1000 dilution.

      The indicated qualtities of human IgG were spotted. Blocking was performed for 1 hour at room temperature using a proprietary blocking buffer prior to addition of ab6858.

       

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (22)

    Publishing research using ab6858? Please let us know so that we can cite the reference in this datasheet.

    ab6858 has been referenced in 22 publications.

    • Niu X  et al. Longitudinal analysis of the antibody repertoire of a Zika virus-infected patient revealed dynamic changes in antibody response. Emerg Microbes Infect 9:111-123 (2020). PubMed: 31906823
    • Lv Z  et al. CD19-targeting fusion protein combined with PD1 antibody enhances anti-tumor immunity in mouse models. Oncoimmunology 9:1747688 (2020). PubMed: 32363119
    • Mohseni AH  et al. The First Clinical Use of a Recombinant Lactococcus lactis Expressing Human Papillomavirus Type 16 E7 Oncogene Oral Vaccine: A Phase I Safety and Immunogenicity Trial in Healthy Women Volunteers. Mol Cancer Ther 19:717-727 (2020). PubMed: 31645442
    • Hiramatsu S  et al. Gastric cancer cells alter the immunosuppressive function of neutrophils. Oncol Rep 43:251-259 (2020). PubMed: 31746403
    • McElhanon KE  et al. Autoantibodies targeting TRIM72 compromise membrane repair and contribute to inflammatory myopathy. J Clin Invest 130:4440-4455 (2020). PubMed: 32687067
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    Hi, I would like to know whether this Ab cross reacts with IgGs from any other species. If yes, are there any Abs from your company that recognizes specifically human IgGs? Thank you

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    Answer

    Anti-Human IgG antibody ab6858 has not been tested for reactivity with other species. For an antibody that is exclusively reactive with human IgG, we recommend ab97175.

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    Question

                    Hello,   I purchased the Goat polyclonal Secondary Antibody to Human IgG - H&L (HRP) (ab6858). May I ask about the dilution factor for this antibody in ELISA? Thank you very much!   Best regards,  

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    Answer

    Thank you for contacting us. For this secondary antibody we recommend a 1/180K dilution with ELISA. However, you will need to optimise and determine the best dilutions for your specific assay; considering starting dilutions from 1/40000. I hope this information is helpful to you.

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    Question

    I want to purchase goat anti-human IgG secondary antibody conjugated with HRP for ELISA. I want to test the autoantibodies in human serum. What is the difference between pre-adsorbed and none pre-adsorbed antibody? Which one is better for my test? Thank you very much!  Best regards,  

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    Answer

    Thank you for contacting us. Pre-adsorbed antibodies are more specific towards primary antibodies than the non pre-adsorbed products. Usually, a conjugated antibody is purified by affinity chromatography using an antigen of interest coupled to agarose beads. The conjugated antibody binds its antigen on the column and will then be eluted. Pre-adsorption purifications can be performed to further increase the specificity of a secondary antibody. Adsorption chromatography involves a stationary phase as an adsorbent. Conjugated antibodies are then passed through gel containing immobilized serum proteins from potentially cross-reactive species. The resulting purified antibody is more pure and specific significantly reducing cross reactivity. Pre-adsorbed secondary antibodies reduce non-specific background staining, especially in IHC when working with closely homologous species I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Is there any difference between Human IgG antibodies ab6858 and ab7153?

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    Answer

    Thank you for your recent telephone enquiry. I can confirm that these two antibodies are essentially the same. The difference is that ab7153 is further purified by preadsorbtion to minimize its cross reactivity to 10 serum other serum proteins. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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