Goat Anti-Human IgG H&L (HRP) (ab6858)

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Overview

  • Product name
    Goat Anti-Human IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Human IgG - H&L (HRP)
  • Host species
    Goat
  • Target species
    Human
  • Tested applications
    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    human IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/5000 - 1/25000.

Images

  • Dot Blot - Goat Anti-Human IgG H&L (HRP) (ab6858)
    Dot Blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

    Dot blot analysis of human IgG using ab6858 at 1/1000 dilution.

    The indicated qualtities of human IgG were spotted. Blocking was performed for 1 hour at room temperature using a proprietary blocking buffer prior to addition of ab6858.

     

References

This product has been referenced in:
See all 9 Publications for this product

Publishing research using ab6858? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

Hi, I would like to know whether this Ab cross reacts with IgGs from any other species. If yes, are there any Abs from your company that recognizes specifically human IgGs? Thank you

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Answer

Anti-Human IgG antibody ab6858 has not been tested for reactivity with other species. For an antibody that is exclusively reactive with human IgG, we recommend ab97175.

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Question

                Hello,   I purchased the Goat polyclonal Secondary Antibody to Human IgG - H&L (HRP) (ab6858). May I ask about the dilution factor for this antibody in ELISA? Thank you very much!   Best regards,  

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Answer

Thank you for contacting us. For this secondary antibody we recommend a 1/180K dilution with ELISA. However, you will need to optimise and determine the best dilutions for your specific assay; considering starting dilutions from 1/40000. I hope this information is helpful to you.

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Question

I want to purchase goat anti-human IgG secondary antibody conjugated with HRP for ELISA. I want to test the autoantibodies in human serum. What is the difference between pre-adsorbed and none pre-adsorbed antibody? Which one is better for my test? Thank you very much!  Best regards,  

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Answer

Thank you for contacting us. Pre-adsorbed antibodies are more specific towards primary antibodies than the non pre-adsorbed products. Usually, a conjugated antibody is purified by affinity chromatography using an antigen of interest coupled to agarose beads. The conjugated antibody binds its antigen on the column and will then be eluted. Pre-adsorption purifications can be performed to further increase the specificity of a secondary antibody. Adsorption chromatography involves a stationary phase as an adsorbent. Conjugated antibodies are then passed through gel containing immobilized serum proteins from potentially cross-reactive species. The resulting purified antibody is more pure and specific significantly reducing cross reactivity. Pre-adsorbed secondary antibodies reduce non-specific background staining, especially in IHC when working with closely homologous species I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Is there any difference between Human IgG antibodies ab6858 and ab7153?

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Answer

Thank you for your recent telephone enquiry. I can confirm that these two antibodies are essentially the same. The difference is that ab7153 is further purified by preadsorbtion to minimize its cross reactivity to 10 serum other serum proteins. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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