Overview

  • Product name

    Goat Anti-Human IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Human
  • Specificity

    No crossreaction with human IgM and IgA in ELISA No crossreaction with albumin fraction of human serum. No crossreaction with albumin fraction of other animal species.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Immunogen

    Human IgG.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.50
    Constituents: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
  • Conjugation notes

    The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel filtation and ion exchange chromatography to clear unbound conjugate.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100.
ELISA 1/1000.

References

This product has been referenced in:

  • Londono-Renteria BL  et al. Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in Colombia. Front Public Health 6:111 (2018). Read more (PubMed: 29868532) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us with your questions. Ab81202 was raised against human IgG, and since ab81202 is polyclonal, I expect it to react with both the heavy and light chains. I do not think that this has been specifically tested. Ab81202 has been tested for reactivity against human albumin and there was no cross-reactivity found. We do not have a distributor into Norway, and our products ship directly into your country. Ab81202 is currently available and, if ordered today, would be expected to arrive by Thursday, May 19. I hope this information is helpful, but please let me know if you have any further questions.

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