Key features and details
- Goat Anti-Human IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ELISA
Product nameGoat Anti-Human IgG H&L (HRP)
See all IgG secondary antibodies
SpecificityNo crossreaction with human IgM and IgA in ELISA No crossreaction with albumin fraction of human serum. No crossreaction with albumin fraction of other animal species.
Tested applicationsSuitable for: WB, ELISAmore details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.50
Constituents: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesFrom the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
Conjugation notesThe purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel filtation and ion exchange chromatography to clear unbound conjugate.
Our Abpromise guarantee covers the use of ab81202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab81202 has been referenced in 2 publications.
- Maldonado-Ruiz LP et al. Differential Tick Salivary Protein Profiles and Human Immune Responses to Lone Star Ticks (Amblyomma americanum) From the Wild vs. a Laboratory Colony. Front Immunol 10:1996 (2019). PubMed: 31555263
- Londono-Renteria BL et al. Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in Colombia. Front Public Health 6:111 (2018). PubMed: 29868532