Goat Anti-Human IgG H&L (HRP) preadsorbed (ab7153)

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Key features and details

  • Goat Anti-Human IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, WB

Overview

  • Product name

    Goat Anti-Human IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Human

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.

  • Tested applications

    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details

  • Immunogen

    Full length native human IgG (purified).

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Horseradish Peroxidase (HRP)

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/500 - 1/2500.
Dot blot
Use at an assay dependent dilution.
ELISA
1/7000 - 1/200000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
Notes
ICC/IF
1/500 - 1/2500.
Dot blot
Use at an assay dependent dilution.
ELISA
1/7000 - 1/200000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (3)

Publishing research using ab7153? Please let us know so that we can cite the reference in this datasheet.

ab7153 has been referenced in 3 publications.

  • Bell BN  et al. Neutralizing antibodies targeting the SARS-CoV-2 receptor binding domain isolated from a naïve human antibody library. Protein Sci 30:716-727 (2021). PubMed: 33586288
  • Uppu DSSM  et al. Temporal release of a three-component protein subunit vaccine from polymer multilayers. J Control Release 317:130-141 (2020). PubMed: 31756392
  • Henson D  et al. Association Between ApoA-I (Apolipoprotein A-I) Immune Complexes and Adverse Cardiovascular Events-Brief Report. Arterioscler Thromb Vasc Biol 39:1884-1892 (2019). PubMed: 31315438

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Dear Tech Support Team,

Kindly advise if the following options of the secondary ab will work in Elisa settings to quantify interaction with the attached primary mAb (it is IgG3 λ chain ):

https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Human-IgG-H-L-HRP-pre-adsorbed-ab102429.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Human-IgG-HL-HRP-pre-adsorbed-ab7153.html

Thanks in advance for your assistance.

Have a nice week,

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Answer

Thank you for contacting us and your interest in our products.
Both of the secondary antibodies chosen (ab102429 and ab7153) should be able to detect the primary antibody (human IgG3) in the ELISA the customer is hoping to perform.
I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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Question

Is there any difference between Human IgG antibodies ab6858 and ab7153?

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Answer

Thank you for your recent telephone enquiry. I can confirm that these two antibodies are essentially the same. The difference is that ab7153 is further purified by preadsorbtion to minimize its cross reactivity to 10 serum other serum proteins. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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Question

Does this antibody recognize all IgG subtypes?

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Answer

Thank you for your enquiry. These antibodies should recognize all human IgG subclasses. Please contact us again if you require further information.

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