Question (69620) | Goat Anti-Human IgG H&L (HRP) preadsorbed (ab7153)

Go to datasheet (ab7153)

Question

Dear Tech Support Team,

Kindly advise if the following options of the secondary ab will work in Elisa settings to quantify interaction with the attached primary mAb (it is IgG3 λ chain ):

https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Human-IgG-H-L-HRP-pre-adsorbed-ab102429.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Human-IgG-HL-HRP-pre-adsorbed-ab7153.html

Thanks in advance for your assistance.

Have a nice week,

Answer

Thank you for contacting us and your interest in our products.
Both of the secondary antibodies chosen (ab102429 and ab7153) should be able to detect the primary antibody (human IgG3) in the ELISA the customer is hoping to perform.
I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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