Product nameGoat Anti-Human IgG H&L preadsorbed
See all IgG secondary antibodies
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to monkey IgG was detected.
Tested applicationsSuitable for: WB, ELISA, ICC, IHCmore details
Human IgG-heavy and light chain
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was cross adsorbed using a monkey immunosorbent to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads
Our Abpromise guarantee covers the use of ab209700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/100000.|
|ELISA||1/1000 - 1/20000.|
|ICC||1/200 - 1/2000.|
|IHC||1/200 - 1/2000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab209700 has not yet been referenced specifically in any publications.