Key features and details
- Goat polyclonal Secondary Antibody to Human IgG - H&L, pre-adsorbed
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Product nameGoat Anti-Human IgG H&L preadsorbed
See all IgG secondary antibodies
DescriptionGoat polyclonal Secondary Antibody to Human IgG - H&L, pre-adsorbed
By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, mouse, pig, rabbit and rat was detected.
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Chicken, Cow, Horse, Mouse, Pig, Rabbit, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was cross adsorbed using bovine, chicken, horse, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Our Abpromise guarantee covers the use of ab97166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/200 - 1/2000.|
|ELISA||1/100 - 1/10000. ELISA-Coating 1/100 - 1/500; ELISA-Primary 1/1000 - 1/10000|
|WB||1/1000 - 1/30000. WB-Colorimetric 1/1000 - 1/10000; WB-Chemiluminescent 1/1000 - 1/30000|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97166 has been referenced in 1 publication.
- Boysen L et al. Generic immune complex assay for detection of murine anti-drug-antibodies in complex with human IgG. Biologicals 60:42-48 (2019). PubMed: 31200984