Key features and details
- Goat Anti-Human IgM H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Suitable for: ICC/IF
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesFrom the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgM. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgM a minimum of 65% protein bound.
Conjugation notesThe purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
Light chain typeunknown
General notesF/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgM. There is low background fluorescence in normal tissue in contrast to cases of auto immune desease where IgM is depostited.
Our Abpromise guarantee covers the use of ab8497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab8497 has not yet been referenced specifically in any publications.