Key features and details
- Goat Anti-Human IgM mu chain (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Product nameGoat Anti-Human IgM mu chain (HRP)
See all IgM secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgM. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
Our Abpromise guarantee covers the use of ab97205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/200 - 1/2000.|
|ELISA||1/10000 - 1/100000. (as Primary).|
|WB||1/2000 - 1/25000. Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97205 has been referenced in 1 publication.
- Stockdale L et al. HIV, HCMV and mycobacterial antibody levels: a cross-sectional study in a rural Ugandan cohort. Trop Med Int Health 24:247-257 (2019). PubMed: 30506614