Goat Anti-Human IgM mu chain (HRP) preadsorbed (ab98549)
Key features and details
- Goat polyclonal Secondary Antibody to Human IgM - mu chain (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ELISA, WB, ICC
Overview
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Product name
Goat Anti-Human IgM mu chain (HRP) preadsorbed
See all IgM secondary antibodies -
Description
Goat polyclonal Secondary Antibody to Human IgM - mu chain (HRP), pre-adsorbed -
Host species
Goat -
Target species
Human -
Specificity
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgM was detected. This antibody may cross react with IgM from other species. -
Tested applications
Suitable for: IHC-P, ELISA, WB, ICCmore details -
Minimal
cross-reactivity
Mouse, Rat more details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
Applications
Our Abpromise guarantee covers the use of ab98549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/2000. | |
| ELISA | 1/10000 - 1/100000. (Primary) | |
| WB | 1/2000 - 1/25000. (Colorimetric: 1/2,000-1/20,000; Chemiluminescent: 1/5,000-1/25,000). | |
| ICC | Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab98549 has not yet been referenced specifically in any publications.
