Key features and details
- Goat polyclonal Secondary Antibody to Human IgM - mu chain (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ELISA, WB, ICC
Product nameGoat Anti-Human IgM mu chain (HRP) preadsorbed
See all IgM secondary antibodies
DescriptionGoat polyclonal Secondary Antibody to Human IgM - mu chain (HRP), pre-adsorbed
SpecificityAntiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgM was detected. This antibody may cross react with IgM from other species.
Tested applicationsSuitable for: IHC-P, ELISA, WB, ICCmore details
Mouse, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP.
Our Abpromise guarantee covers the use of ab98549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/2000.|
|ELISA||1/10000 - 1/100000. (Primary)|
|WB||1/2000 - 1/25000. (Colorimetric: 1/2,000-1/20,000; Chemiluminescent: 1/5,000-1/25,000).|
|ICC||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab98549 has not yet been referenced specifically in any publications.