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Number of blots:
At least 20 blots (based on a 1 µl/ml dilution in 5 ml milk).
Important protocol notes:
1. The anti-goat VeriBlot for IP secondary antibody (HRP) detects goat IgG antibodies.
2. The anti-goat VeriBlot for IP secondary antibody (HRP) preferentially detects the non-reduced form of goat IgG over the reduced, SDS-denatured forms.
3. IP sample should be completely reduced/denatured before loaded onto a western blot.
4. Milk should be used as the blocking protein for the immunoblot.
Western blot and IP resources:
a) Western blot a beginner's guide
b) IP protocol
c) IP troubleshooting tips
Our Abpromise guarantee covers the use of ab157532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. The dilution will depend on the sensitivity of the HRP substrate. The recommended dilution is 1 µl in 1 ml milk. Based on the recommended dilution in 5 ml milk researchers can perform 20 western blots. This product is recommended for the western blot detection of IP samples.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"