Goat IgG, polyclonal - Isotype Control (ab37373)

Q&A (5)References (14)

Overview

  • Product name
    Goat IgG, polyclonal - Isotype Control
    See all Goat isotype controls
  • Specificity
    This antibody has been selected as an isotype control as it has no known specificity.
  • Tested applications
    Suitable for: ELISA, Flow Cyt, ChIP/Chipmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • Purity
    Affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas
  • Alternative names
    • Goat Isotype Control

Applications

Our Abpromise guarantee covers the use of ab37373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.
ChIP/Chip Use at an assay dependent concentration. PubMed: 20336405

References

This product has been referenced in:
See all 14 Publications for this product

Publishing research using ab37373? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Q&A
Submit a question

1-5 of 5 Q&A

Question

Please advise if ab37373 is suitable isotype control for ab11503 (which is a mix of subclasses).

Thank you in advance.

Have a nice week,

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Answer

Thank you for contacting us.
The Isotype control ab37373 can be used with ab11503.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question


Thank you for you e-mail.
I would like to purchase ab37373 and participate in the mentioned below
scheme. First of all, in our institute ordering system we need invioce
before ordering the product. Therefore, I would be very grateful if you
could prepare the invoice. And I have a few questions concerning this
scheme. Do my results have to be published in article and then I will have
a right to order something? How much time will I get to perform an
experiment and send my results? What kind of documents and photos do you
need?
Best regards,

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Answer

Thank you fro getting back to me.

I'm glad to hear you would like to take part in the testing discount scheme with ab37373. All you need to do is purchase ab37373, test it with your IHC samples and submit an Abreview of the results. Regardless of whether you find it a good control in such an experiment or not you will then be eligible to claim the value of ab37373 off your next order with us. The only constraint to the offer is that you much purchase ab37373, test it, submit the Abreview and claim your discount within a 4 month period.

I have generated the discount code for you as follows:

xxxxx

This code must be added to the "Additional comments" section when submitting your Abreview. Once the review has been submitted the review will then be active and you can claim the value of ab37373 off your next order by quoting the code when placing the order.

If you have any questions in regards to this please do let me know. More details about the testing discount scheme can be found from the following page:

https://www.abcam.com/collaboratordiscount

As requested, I have generated the proforma for you which have been sent in a separate email.

I hope this information has been of help. If you require anything else please do let me know.

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Question

Dear Sirs,
I would like to buy a product 'Goat Control IgG (ab37373)' which I will
use to carry out Chip on Chip method. However, I would like to know if
there is a possibility to use this product as a negative control in
immunohistochemical/immunocytochemical stainings. Do you have any
information about applications in mentioned techniques?
Sincerely,

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Answer

Thank you for contacting us.

I'm glad that you would like to use the Goat Control IgG (ab37373) to work as a control in your ChIP/ChIP experiments. We have not as yet ourselves used this antibody as a control in IHC but I see no reason why it would not work well in this application. However, as we have no data to support this I would be unable to guarantee this.

If you would be willing to purchase ab37373, share the results obtained with us bysubmit an Abreview with its use in IHC as a control you would be eligible for a testing discount. This would mean that you could claim the equivalent price of ab37373 off any product in our catalogue. More information on this offer can be found from the following link:

https://www.abcam.com/collaboratordiscount

The discount code required needs to be raised prior to purchase ofab37373 so please do let me know if you would like to participate in this scheme.

I hope this information has been of help. If you require any further information please do not hesitate to ask.

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Question

dear correspondent,

We have lately purchased several Abs from Abcam and i have a question related to the purification method.
In the datasheets of several monoclonal antibodies is mentioned that they are purified as 'IgG fraction'. Could you exlain to me what that means? Is a anti-IgG column used or an protein A/G column (or any other technique)? This is very important information to us, as we need to consider protein A/G contamination to explain data obtained in our R&D dept. in experiments using these antibodies.

More specifically, the question relates to the following antibodies (data sheets of all those abs mention 'IgG fraction' in 'purity' annotation):

ab3609-100 (anti-Aurora B)
ab51841-100 (anti-TATA binding protein)
ab37373 (goat control IgG)
ab46540 (rabbit control IgG)


Thanks in advance and hope to hear your answer soon,

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Answer

Thank you for your enquiry.

I have confirmed the purification details of the antibodies you listed and have updated the datasheets where necessary:

ab3609 (Aurora B)
Protein G purified

ab51841 (TATA binding protein)
Protein G purified

ab37373 (goat control IgG)
Proprietary combination of ammonium sulfate precipitation, ion exchange, and/or other chromatography techniques.

ab46540 (rabbit control IgG)
Affinity purified against the antigen.

I hope this will be helpful. If you require any further information on the purification of other products, or any other questions, please do not hesitate to contact me.

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Question

2. which method was useto obtain the ab37373? (salting out or other)?

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Answer

ab37373 was purified usinga proprietary combination of ammonium sulfate precipitation, ion exchange, and/or other chromatography techniques. I'm sorry I can not be more specific in this case.

I hope this is helpful. Please contact me again if you have any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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