Recombinant

Recombinant Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (ab238530)

Overview

  • Product name

    Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin)
  • Host species

    Goat
  • Target species

    Rabbit
  • Specificity

    ab238530 reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Minimal
    cross-reactivity


    Human, Mouse, Rat more details
  • Immunogen

    Full length protein corresponding to Rabbit IgG.

  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab238530 was purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RMG01
  • Isotype

    IgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab238530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml.

Non-reduced

ELISA Use a concentration of 0.01 - 0.5 µg/ml.

Images

  • All lanes : Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (ab238530) at 0.2 µg/ml

    Lane 1 : Non-reduced rabbit IgG
    Lane 2 : Reduced rabbit IgG

    Lysates/proteins at 0.02 µg per lane.
  • ELISA of IgGs from different species, shows ab238530 reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. ab238530 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

  • A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of ab238530 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

References

ab238530 has not yet been referenced specifically in any publications.

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