Recombinant

Recombinant Goat monoclonal [RMG02] Anti-Rabbit IgG Fc (Biotin) (ab238531)

Overview

  • Product name

    Goat monoclonal [RMG02] Anti-Rabbit IgG Fc (Biotin)
  • Host species

    Goat
  • Target species

    Rabbit
  • Specificity

    ab238531 reacts to the Fc region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.

  • Tested applications

    Suitable for: ELISA, WBmore details
  • Minimal
    cross-reactivity


    Human, Mouse, Rat more details
  • Immunogen

    Full length protein corresponding to Rabbit IgG.

  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab238531 was purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RMG02
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab238531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.01 - 0.5 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Use under non reducing condition. Predicted molecular weight: 150 kDa.

Images

  • All lanes : Goat monoclonal [RMG02] Anti-Rabbit IgG Fc (Biotin) (ab238531) at 0.2 µg/ml

    Lane 1 : Non-reduced rabbit IgG
    Lane 2 : Reduced rabbit IgG

    Lysates/proteins at 0.02 µg per lane.

    Predicted band size: 150 kDa

  • ELISA of IgGs from different species shows ab238531 reacts to the Fc region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. ab238531 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

  • A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of ab238531 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

References

ab238531 has not yet been referenced specifically in any publications.

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