Key features and details
- Goat monoclonal [RMG02] Anti-Rabbit IgG Fc (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, WB
Product nameGoat monoclonal [RMG02] Anti-Rabbit IgG Fc (Biotin)
ab238531 reacts to the Fc region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.
Tested applicationsSuitable for: ELISA, WBmore details
Human, Mouse, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length protein corresponding to Rabbit IgG.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
Purification notesab238531 was purified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab238531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.01 - 0.5 µg/ml.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Use under non reducing condition. Predicted molecular weight: 150 kDa.|
All lanes : Goat monoclonal [RMG02] Anti-Rabbit IgG Fc (Biotin) (ab238531) at 0.2 µg/ml
Lane 1 : Non-reduced rabbit IgG
Lane 2 : Reduced rabbit IgG
Lysates/proteins at 0.02 µg per lane.
Predicted band size: 150 kDa
ELISA of IgGs from different species shows ab238531 reacts to the Fc region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. ab238531 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of ab238531 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238531 has not yet been referenced specifically in any publications.