Recombinant Goat monoclonal [RMG02] Anti-Rabbit IgG Fc (Biotin) (ab238531)
Key features and details
- Goat monoclonal [RMG02] Anti-Rabbit IgG Fc (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, WB
Overview
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Product name
Goat monoclonal [RMG02] Anti-Rabbit IgG Fc (Biotin) -
Host species
Goat -
Target species
Rabbit -
Specificity
ab238531 reacts to the Fc region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.
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Tested applications
Suitable for: ELISA, WBmore details -
Minimal
cross-reactivity
Human, Mouse, Rat more details -
Immunogen
Full length protein corresponding to Rabbit IgG Fc.
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab238531 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RMG02 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use a concentration of 0.01 - 0.5 µg/ml.
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Use under non reducing condition. Predicted molecular weight: 150 kDa.
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Notes |
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ELISA
Use a concentration of 0.01 - 0.5 µg/ml. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Use under non reducing condition. Predicted molecular weight: 150 kDa. |
Images
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All lanes : Goat monoclonal [RMG02] Anti-Rabbit IgG Fc (Biotin) (ab238531) at 0.2 µg/ml
Lane 1 : Non-reduced rabbit IgG
Lane 2 : Reduced rabbit IgG
Lysates/proteins at 0.02 µg per lane.
Predicted band size: 150 kDa -
ELISA of IgGs from different species shows ab238531 reacts to the Fc region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. ab238531 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of ab238531 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab238531 has not yet been referenced specifically in any publications.