Overview

  • Product name

    Goat Anti-Mouse IgA alpha chain
    See all IgA secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications

    Suitable for: IHC-P, ELISA, WB, ICCmore details
  • Immunogen

    Other Immunogen Type corresponding to Mouse IgA.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/2000.
ELISA 1/100 - 1/30000. Coating: 1/100 - 1/500, Primary: 1/1000 - 1/30000
WB 1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000, Chemiluminescent: 1/1000 - 1/30000
ICC Use at an assay dependent dilution.

References

This product has been referenced in:

  • Emgård J  et al. Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. Immunity 48:120-132.e8 (2018). Read more (PubMed: 29343433) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

The whole molecule IgA was injected into the animal. The light chain antibody was then adsorbed out of the serum before the affinity purification, leaving with the heavy chain only.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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