Goat Anti-Mouse IgA alpha chain (ab97231)
Key features and details
- Goat Anti-Mouse IgA alpha chain
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ELISA, WB, ICC
Related conjugates and formulations
Overview
-
Product name
Goat Anti-Mouse IgA alpha chain
See all IgA secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. -
Tested applications
Suitable for: IHC-P, ELISA, WB, ICCmore details -
Immunogen
Other Immunogen Type corresponding to Mouse IgA.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
1/200 - 1/2000.
|
ELISA |
1/100 - 1/30000. Coating: 1/100 - 1/500, Primary: 1/1000 - 1/30000
|
|
WB |
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000, Chemiluminescent: 1/1000 - 1/30000
|
|
ICC |
Use at an assay dependent dilution.
|
Notes |
---|
IHC-P
1/200 - 1/2000. |
ELISA
1/100 - 1/30000. Coating: 1/100 - 1/500, Primary: 1/1000 - 1/30000 |
WB
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000, Chemiluminescent: 1/1000 - 1/30000 |
ICC
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab97231 has been referenced in 6 publications.
- Su F et al. Early Oral Administration of Ginseng Stem-Leaf Saponins Enhances the Peyer's Patch-Dependent Maternal IgA Antibody Response to a PEDV Inactivated Vaccine in Mice, with Gut Microbiota Involvement. Vaccines (Basel) 11:N/A (2023). PubMed: 37112742
- Qin D et al. Secretory IgA-ETEC F5 Immune Complexes Promote Dendritic Cell Differentiation and Prime T Cell Proliferation in the Mouse Intestine. Life (Basel) 13:N/A (2023). PubMed: 37763339
- Murai A et al. Class II lupus nephritis with podocyte injury in imiquimod-induced lupus-prone mice. Histol Histopathol 37:655-664 (2022). PubMed: 35297032
- Mei L et al. Lactobacillus fermentum Stimulates Intestinal Secretion of Immunoglobulin A in an Individual-Specific Manner. Foods 11:N/A (2022). PubMed: 35563952
- Petrova T et al. Prevention and partial reversion of the lupus phenotype in ABIN1[D485N] mice by an IRAK4 inhibitor. Lupus Sci Med 8:N/A (2021). PubMed: 34836923
- Emgård J et al. Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. Immunity 48:120-132.e8 (2018). PubMed: 29343433