Goat Anti-Mouse IgA alpha chain (DyLight® 650) (ab97014)
Key features and details
- Goat Anti-Mouse IgA alpha chain (DyLight® 650)
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Goat Anti-Mouse IgA alpha chain (DyLight® 650)
See all IgA secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Immunogen
Other Immunogen Type corresponding to Mouse IgA.
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Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 650 replaces DyLight® 649. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
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ICC/IF |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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Notes |
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IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab97014 has been referenced in 3 publications.
- Schmidt H et al. Protein-based condensation mechanisms drive the assembly of RNA-rich P granules. Elife 10:N/A (2021). PubMed: 34106046
- Jash A et al. ZBTB32 restrains antibody responses to murine cytomegalovirus infections, but not other repetitive challenges. Sci Rep 9:15257 (2019). PubMed: 31649328
- Planer JD et al. Development of the gut microbiota and mucosal IgA responses in twins and gnotobiotic mice. Nature 534:263-6 (2016). PubMed: 27279225