Key features and details
- Goat Anti-Mouse IgA alpha chain (DyLight® 650)
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Product nameGoat Anti-Mouse IgA alpha chain (DyLight® 650)
See all IgA secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
Other Immunogen Type corresponding to Mouse IgA.
ConjugationDyLight® 650. Ex: 654nm, Em: 673nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
General notesDyLight® 650 replaces DyLight® 649.
Our Abpromise guarantee covers the use of ab97014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.
|ICC/IF||1/50 - 1/500.|
|Flow Cyt||1/50 - 1/200.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97014 has been referenced in 2 publications.
- Jash A et al. ZBTB32 restrains antibody responses to murine cytomegalovirus infections, but not other repetitive challenges. Sci Rep 9:15257 (2019). PubMed: 31649328
- Planer JD et al. Development of the gut microbiota and mucosal IgA responses in twins and gnotobiotic mice. Nature 534:263-6 (2016). PubMed: 27279225