Goat Anti-Mouse IgA alpha chain (FITC) (ab97234)

Submit a review Q&A (1)References (3)

Key features and details

  • Goat Anti-Mouse IgA alpha chain (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, Flow Cyt

Overview

  • Product name

    Goat Anti-Mouse IgA alpha chain (FITC)
    See all IgA secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details

  • Immunogen

    Other Immunogen Type corresponding to Mouse IgA.

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.

  • Conjugation notes

    F/P ratio is 3-7

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab97234 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P 1/50 - 1/500.
    ICC/IF 1/50 - 1/500.
    Flow Cyt 1/50 - 1/200.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (3)

    Publishing research using ab97234? Please let us know so that we can cite the reference in this datasheet.

    ab97234 has been referenced in 3 publications.

    • Hu X  et al. Respiratory Syncytial Virus Exacerbates Kidney Damages in IgA Nephropathy Mice via the C5a-C5aR1 Axis Orchestrating Th17 Cell Responses. Front Cell Infect Microbiol 9:151 (2019). PubMed: 31134161
    • Jokl EJ  et al. Transcriptional upregulation of Bag3, a chaperone-assisted selective autophagy factor, in animal models of KY-deficient hereditary myopathy. Dis Model Mech 11:N/A (2018). PubMed: 29914939
    • Xia F  et al. IRGM1 regulates oxidized LDL uptake by macrophage via actin-dependent receptor internalization during atherosclerosis. Sci Rep 3:1867 (2013). PubMed: 23689639

    Customer reviews and Q&As

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    Question

    Can Goat polyclonal Secondary Antibody to Mouse IgA - alpha chain (FITC) (ab97234) be used as seondary antibody for "Anti-Francisella tularensis LPS antibody [FB11] (ab2033) in indirect immunofluorescence assay?

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    Answer

    Thank you for your inquiry.


    We would not recommend ab97234 as a secondary for ab2033 because they do not match in isotype. The secondary ab97234 targets Mouse IgA, but the primary ab2033 is IgG2a.


    I would reccomend using ab6785 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) instead.


    https://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-ab6785.html


    I hope this information helps. Please contact us with any other questions.

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