Overview

  • Product name

    Goat Anti-Mouse IgA alpha chain (HRP)
    See all IgA secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. Cross reactivity to mouse IgM, IgG1, IgG2a, IgG2b, IgG2c, IgG3 and IgE is undetectable. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Immunogen

    Other Immunogen Type corresponding to Mouse IgA.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.05% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes

    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (primary).
WB 1/2000 - 1/25000. Colorimetric: 1/2000 - 1/20000, Chemiluminescent: 1/5000 - 1/25000

References

This product has been referenced in:

  • Liu C  et al. The mucosal adjuvant effect of plant polysaccharides for induction of protective immunity against Helicobacter pylori infection. Vaccine 37:1053-1061 (2019). Read more (PubMed: 30665774) »
  • Mohseni AH  et al. Extracellular overproduction of E7 oncoprotein of Iranian human papillomavirus type 16 by genetically engineered Lactococcus lactis. BMC Biotechnol 19:8 (2019). Read more (PubMed: 30678667) »
See all 10 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

As requested, I have issued a free of charge replacement with the order number XXXXX for a new unit of ab98688.

To check the status of the order please contact our Customer Service team and reference this number. You will soon receive a confirmation informing about the delivery date.

I hope this kit gives the expected results. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to investigate this particular case further for you, and also obtain more information for our quality records.

In order to verify the HRP enzyme has not been degraded, I would like to ask you to please add some drops of secondary directly on the membrane, followed by addition of the substrate.

If no colour is developed, the lack of signal may be due to enzyme degradation. Otherwise, some further problem with the antibody’s specificity may be occurring.

I assume you have checked the isotypes are compatible, and you have tried incubating the secondary with other primaries to dismiss primary failure.

Please note that all of our products are covered by our Abpromise guarantee; which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

I look forward for receiving your reply.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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