Key features and details
- Goat Anti-Mouse IgE H&L
- Host species: Goat
- Suitable for: ELISA, Immunodiffusion, Immunoelectrophoresis, WB
Product nameGoat Anti-Mouse IgE H&L
See all IgE secondary antibodies
SpecificityReacts only with IgE by IEP, gel diffusion and ELISA.
Tested applicationsSuitable for: ELISA, Immunodiffusion, Immunoelectrophoresis, WBmore details
Goats were immunized with a highly purified mouse IgE hybridoma protein in CFA
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: 0.194% Methyl mannoside, 0.812% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antiserum was solid phase absorbed to ensure class specificity. The anti epsilon antibodies were immunoaffinity purified off an immunoabsorbant containing mouse IgE protein different from that used for immunization.
Our Abpromise guarantee covers the use of ab9162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|Immunodiffusion||Use at an assay dependent concentration.|
|Immunoelectrophoresis||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab9162 has been referenced in 2 publications.
- Santiago Hda C et al. Helminth infection alters IgE responses to allergens structurally related to parasite proteins. J Immunol 194:93-100 (2015). PubMed: 25404363
- McNeil BD et al. Identification of a mast-cell-specific receptor crucial for pseudo-allergic drug reactions. Nature 519:237-41 (2015). PubMed: 25517090