Overview

  • Product name

    Goat Anti-Mouse IgG Fc (FITC)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG-Fc. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Conjugation notes

    F/P ratio is 4.4
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • A431 cell nucleus (DAPI signal) showing three gamma-H2AX foci structures.
    Primary anitibody H2A.X (phospho S139) antibody [3F2], ab22551, 100ug.
    Secondary antibody Mouse IgG-Fc (FITC), ab97264, 1mg.

References

This product has been referenced in:

  • Yu X  et al. Complex Interplay between Epitope Specificity and Isotype Dictates the Biological Activity of Anti-human CD40 Antibodies. Cancer Cell 33:664-675.e4 (2018). Read more (PubMed: 29576376) »
  • Han Z  et al. Targeted gadofullerene for sensitive magnetic resonance imaging and risk-stratification of breast cancer. Nat Commun 8:692 (2017). Read more (PubMed: 28947734) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thanks for sending the purchase order information. I have located the order for ab97264. Would you like me to send another vial of this same product as the replacement?

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Answer

Thanks for the reply. So I can certainly set up the replacement to send one vial of ab6724. For some reason the purchase order information did not make it through. If you could, please re-send this and I'll be happy to set up the replacement order. Thank you!

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Answer

Since the antibody is not working as expected I would be happy to send a replacement. I just need a bit more information to process your request: 1. What antibody would you like as the replacement? 2. What is the order number used to purchase ab97264? Thanks for the additional information.

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Answer

You are correct that if we find the antibody is not peforming as expected, based on information on our online datasheet, we would be happy to offer a replacement, credit or refund. In this case, the antibody may be reacting unspecifically. Based on the dilution factor (1:50) the customer notes using in FACS however, I feel that perhaps the customer is using too much antibody. Using too much antibody will produce a high background much the same an antibody which is non-specific. By diluting ab97264 to a working dilution of 1:200, I hope to reduce the background in the experiment. I look forward to hearing if my suggestion proves useful.

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