Overview

  • Product name
    Goat Anti-Mouse IgG Fc (HRP)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    Based on immunoelectrophoresis, reacts with the heavy chains on mouse IgG but not with the light chains on most mouse immunoglobulins. No antibody was detected against mouse IgM, or against non-immunoglobulin serum proteins, but antibodies may cross-react with immunoglobulins from other species.
  • Tested applications
    Suitable for: IHC, ELISA, WBmore details
  • Immunogen

    Mouse IgG Fc fragment.

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.60
    Constituents: 0.164% Sodium phosphate, 1.45% Sodium chloride, 1.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
  • Conjugation notes
    Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC 1/500 - 1/5000.
ELISA 1/5000 - 1/100000.
WB 1/10000 - 1/200000. Predicted molecular weight: 40 kDa.

Images

  • Ab20043 was used at dilution 1/3000 with the primary antibody ab487 in WB. See the review on ab487.

References

This product has been referenced in:
  • Fu W  et al. Tongluo Xingnao effervescent tablet reverses memory deficit and reduces plaque load in APPswe/PS1dE9 mice. Exp Ther Med 15:4005-4013 (2018). Read more (PubMed: 29563991) »
  • Xiao S  et al. Genome-scale RNA interference screen identifies antizyme 1 (OAZ1) as a target for improvement of recombinant protein production in mammalian cells. Biotechnol Bioeng 113:2403-15 (2016). Read more (PubMed: 27215166) »
See all 8 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for contacting us.

Please check the following datasheets as answer to your first question;

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fc-AP-pre-adsorbed-ab49678.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fc-AP-pre-adsorbed-ab49678.html

The antibodies to your second question is

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-HRP-ab97240.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-FITC-ab97239.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-pre-adsorbed-ab98689.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-ab97236.html

For searching the right secondary antibody; please use the secondary search tool on our website.

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Dear Sir/Madam,
We are trying to develop an ELISA for Dengue NS1 detection and since we have been been working for a long time with your products, we have decided to use your dengue products. We are in the process of validating our immunocapture system. The capture antibody we are using is a monoclonal antibody against DEN1-4 NS1 (ab41616) that we purchased together with theDengue NS1 glycoprotein (ab64456). We have already validated the presence of the mouse monoclonalantibody against DEN1-4 NS1 (ab41616) in our ELISA support using a Goat polyclonal Anti-mouse (ab20043). After looking at the citation for themouse monoclonalantibody against DEN1-4 NS1 we are expecting a good immunocapture. However on our initial tries, we cannot detect the presence of the dengue NS1 antigen. So we checked some details in the technical support and we found out that the NS1 antigen has a histidine tag which have been missing in the initial data sheet we had. And so we decided to purchase lasttime a unit of Anti-histidine tag HRP (ab1187) to check on theDengue NS1 glycoprotein (HisTag) (ab64456) but unfortunately even at 2 ug/ml concentration, we are not able to detect its presence. We are thinking of the possibility that the antigen we received may not have histidine tag as initially stated in the product sheet? but as you have guaranteed on our previous inquiry, the antigen is containing histidine tag. As loyal costumers of your company, we would like to ask you for the possibility of requesting replacement of the dengue NS1 glycoprotein (ab64456) that we have initially purchased with a different batch preferably to proceed with our experiments.
We are looking forward in working further with your dengue products.
Thank you for your usual costumer support.
Regards,

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Answer

Thank you for contacting us.
I am sorry to hear you are having problems with one of our products. I appreciate the details provided. I am afraid that product ab41616 is not guaranteed to work in ELISA. We have tested this antibody in WB and ICC, but we are not aware of its suitability for ELISA assays.
As far as I understand you are performing an indirect ELISA, coating your plate with the antigen ab64456 and then capturing it with the monoclonal antibody, using ab20043 (secondary) to detect your primary, is that correct? It could well be the primary antibody is not recognising ab64456 as it may not work for this application.
I can also confirm the peptide ab64456 is tagged with 6 histidines. It is also possible the antibody is not detecting this protein if the epitope it recognizes is masked with the histidine tags.
If you wish to I can try to find out the epitope targeted by ab41616 and check whether it is comprised within the tag region.
Regarding the failure of ab1187 (Anti-6X His tag® antibody (HRP)) to detect the glycoprotein, it could be due to the presence of sodium azide to preserve the protein. The antibody is linked to HRP and this enzyme is very sensitive to sodium azide.
In any case, as all of our products are covered by our Abpromise guarantee, if you wish to receive a replacement for ab64456, I will be more than happy to send it to you.
However, if you want to receive the same protein, you may still experience the same problems. In order to target this protein, I can try to find within our catalogue a primary against it that does not detect the epitope within the tagged sequence, or any other solution you may prefer.
I look forward to receiving your reply.

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Answer

Thank you for your reply. We do not provide samples of our antibodies but under our Abpromise we do guarantee our products to work as stated on the datasheet. If they fail to perform as stated then we will happily replace or refund the antibody. If there is anything else I can do, please let me kn

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Answer

Thank you for contacting Abcam. Based on your requirements, I would recommend ab20043 as it will only react with the heavy chain of the mouse IgG. The image below shows the different regions of the IgG molecule and so from that you will be able to see clearly the regions that the other antibodies you selected bind too.   The difference between the preabsored and non preabsorbed i antibodies s that preabsorbed antibodies reduce non-specific background staining, especially in IHC when working with closely homologous species. If there is anything else I can help you with, please let me know.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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