Overview

  • Product name
    Goat Anti-Mouse IgG Fc (HRP)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - Fc (HRP)
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG-Fc. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes
    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/25000. Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG Fc (HRP) (ab97265)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG Fc (HRP) (ab97265)

    IHC image of beta actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97265,  1/1000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/200 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

This product has been referenced in:
  • McGuire KA  et al. New adenovirus-based vaccine vectors targeting Pfs25 elicit antibodies that inhibit Plasmodium falciparum transmission. Malar J 16:254 (2017). ELISA ; Mouse . Read more (PubMed: 28619071) »
  • El-Diwany R  et al. Extra-epitopic hepatitis C virus polymorphisms confer resistance to broadly neutralizing antibodies by modulating binding to scavenger receptor B1. PLoS Pathog 13:e1006235 (2017). Read more (PubMed: 28235087) »

See all 17 Publications for this product

Publishing research using ab97265? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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9 reviews or Q&A

10
Votes: Highest First


Inquiry: Hi Abcam, I have had great success using this secondary Ab for westerns that involve a IgG1 primary with a film exposure time of only 15s-30s. Just recently I ran a western of a recombinant protein, detecting with an IgG2a primary Ab with...

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Thank you for contacting us.


This antibody should work for all IgG subclasses. Although you know your primary works, perhaps it is somewhat less efficient than the primary antibody with the IgG2a subclass?

I hope this information...

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Hello, Will this product react with all mouse IgG Fc's including mouse IgG1 Fc? Thanks

Thank you for contacting us.

Yes, this antibody will react with the Fc domain of all mouse IgG including IgG1, but not IgA or IgM.

Could I have certificate of analysis of following products please:
Product
Référence
N°lot
Mouse monoconal to Human Serum Albumin
ab10241
3002109900
Mouse monoclonal to Transferrin
ab70826
300210990...

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Thank you for contacting us.

Please find attached certificate of compliance. We do not provide certificate of analysis for antibodies.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any mor...

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Product code: 97265
Lot number: GR78227-1

Inquiry: Hi, Could you send me the certificate of compliance of the product above.

Merci de nous avoir contactés.
Veuillez trouver ci-joint le certificat dont vous avez fait la demande.
J'espère que ce document vous sera utile. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.



wir wären an diesem Angebot sehr interessiert und würden gerne diesen Antikörper testen.

Ich habe letzte Woche schon eine Email an Ihre secondary antibodies product manager geschickt, allerdings noch keine Rückmeldung bezüglich ei...

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Wenn Sie in Zukunft ein Produkt testen möchten, oder allgemin mehr über einen Antikörper oder eines unserer Produkte wissen möchten, wenden Sie sich an uns vom technischen Dienst (wissenschaftlicherdienst@abcam.com). Frau Langenick arbeitet i...

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Dear All,



Sending you attached a list of the tendered items. You can see the products at page 3-5. Please give all of the items a check and let us know which items are best available with Abcam.



Early respo...

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Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please e...

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We have bought the following conjugates from your company. We asked the agancy that we bought about the temperature to keep them to increase the time of keeping. They told us to keep them in -20 C0 and we did for two weeks up to now, but now when we ch...

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Thank you for contacting us.

The datasheet of these antibodies say "Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles"

This mean after receiving the...

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Please let us know regarding the proteins or strain which have been used for below products as desired by one of our customer : ab106292 ab34749 ab108750 ab97265  ( Which H+ or which chain ( Light) ab108750 ( Referance for the same)

Thank you for contacting us.  I am still waiting for additional information regarding the strain used to generate ab106292, but I will let you know as soon as I hear back from the lab regarding this.  The other products are as follows: ab34749: 9B11 wa...

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Do you know what the sequence is that the antibody detects on the IgG Fc part? (Goat anti mouse ab97265)

Thank you for contacting us. Unfortunately I am not able to provide information about the exact antibody binding sequence for ab97265 as this is considered proprietary by the lab that generated the product. As stated on the product specificity, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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