Overview

  • Product name
    Goat Anti-Mouse IgG Fc (HRP)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - Fc (HRP)
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG-Fc. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes
    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/25000. Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000

Images

  • IHC image of beta actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97265,  1/1000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/200 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

This product has been referenced in:
  • Callaghan NI  et al. Cardioprotective mitochondrial binding by hexokinase I is induced by a hyperoxic acute thermal insult in the rainbow trout (Oncorhynchus mykiss). Comp Biochem Physiol B Biochem Mol Biol 224:45-52 (2018). Read more (PubMed: 28723590) »
  • Tripathy A  et al. The molecular connection of histopathological heterogeneity in hepatocellular carcinoma: A role of Wnt and Hedgehog signaling pathways. PLoS One 13:e0208194 (2018). Read more (PubMed: 30513115) »
See all 20 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Answer

Thank you for contacting us.


This antibody should work for all IgG subclasses. Although you know your primary works, perhaps it is somewhat less efficient than the primary antibody with the IgG2a subclass?

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for contacting us.

Yes, this antibody will react with the Fc domain of all mouse IgG including IgG1, but not IgA or IgM.

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Answer

Thank you for contacting us.

Please find attached certificate of compliance. We do not provide certificate of analysis for antibodies.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Merci de nous avoir contactés.
Veuillez trouver ci-joint le certificat dont vous avez fait la demande.
J'espère que ce document vous sera utile. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Answer



Wenn Sie in Zukunft ein Produkt testen möchten, oder allgemin mehr über einen Antikörper oder eines unserer Produkte wissen möchten, wenden Sie sich an uns vom technischen Dienst (wissenschaftlicherdienst@abcam.com). Frau Langenick arbeitet in der Marketingabteilung und leitet Ihre Anfragen zu uns weiter, und da kann es manchmal dauern, bis wir Ihre Anfrage dann bearbeiten können. Allerdings ist secondary antibodies product manager wiederum dafür der richtige Ansprechpartner für Ihre Ergebnisse (obwohl wir die auch bearbeiten können). Ich hoffe, dies ist jetzt nicht zu verwirrend.



DISCOUNT CODE: XXXXXX
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Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab97265 Goat anti-Mouse IgG Fc (HRP) secondary antibody in In-Cell-ELISA (ICE) zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer XXXXXX, deckt den Wert von ab97265 ab, und kann zum Kauf weiterer Produkte eingesetzt werden.

Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in (ICE) eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

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Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

Please find a list of the product code and the links to the respective datasheets below:

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ab6939 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=6939 (or use the following: https://www.abcam.com/index.html?datasheet=6939).

ab96899 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96899 (or use the following: https://www.abcam.com/index.html?datasheet=96899).

ab96919 (Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96919 (or use the following: https://www.abcam.com/index.html?datasheet=96919).

ab96879 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96879 (or use the following: https://www.abcam.com/index.html?datasheet=96879).

ab96871 (Goat anti-Mouse IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96871 (or use the following: https://www.abcam.com/index.html?datasheet=96871).

ab97265 (Goat anti-Mouse IgG Fc (HRP) secondary antibody)

https://www.abcam.com/index.html?datasheet=97265 (or use the following: https://www.abcam.com/index.html?datasheet=97265).

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https://www.abcam.com/index.html?datasheet=7481 (or use the following: https://www.abcam.com/index.html?datasheet=7481).

ab7487 (Rabbit Serum (sterile))

https://www.abcam.com/index.html?datasheet=7487 (or use the following: https://www.abcam.com/index.html?datasheet=7487).

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https://www.abcam.com/index.html?datasheet=46666 (or use the following: https://www.abcam.com/index.html?datasheet=46666).

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https://www.abcam.com/index.html?datasheet=52579 (or use the following: https://www.abcam.com/index.html?datasheet=52579).

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https://www.abcam.com/index.html?datasheet=73430 (or use the following: https://www.abcam.com/index.html?datasheet=73430).

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https://www.abcam.com/index.html?datasheet=116028 (or use the following: https://www.abcam.com/index.html?datasheet=116028).


I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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Answer

Thank you for contacting us.

The datasheet of these antibodies say "Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles"

This mean after receiving the antibody you can keep antibody at 4C for 1-2 weeks when using in your experiments. After you finish your experiment we recomendaliquoting the antibody 5-10 ul in vialsand storing at -20C. We suggest this to avoidthe extra freeze thawing steps, every time you use the antibody.

If you have already stored it at -20C, this is absolutely fine!

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.  I am still waiting for additional information regarding the strain used to generate ab106292, but I will let you know as soon as I hear back from the lab regarding this.  The other products are as follows: ab34749: 9B11 was generated against full-length recombinant Rubella capsid protein expressed in yeast S.cerevisiae. To produce the recombinant antigen, RNA was isolated from Rubella virus strain RA27/3 (GenBank: X14871.1). ab97265: This secondary antibody is specific to the Fc region of Mouse IgG and shows limited cross-reactivity with IgM and IgA. ab108750: The plates used in the kit are coated with Edmonston strain of the measles virus.  I hope this helps, please let me know if you need any additional information. 

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Answer

Thank you for contacting us. Unfortunately I am not able to provide information about the exact antibody binding sequence for ab97265 as this is considered proprietary by the lab that generated the product. As stated on the product specificity, this antibody reacts specifically with Mouse IgG-Fc. Sorry I could not be of more help in this instance. Please do not hesitate to contact us should you have further questions.

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